Zhao Liling, Zhong Jing, Zhang Xiaoyun, Ding Ming, Zhang Zhongkai
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key laboratory of Agricultural Biotechnology, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People's Republic of China.
Arch Virol. 2018 Jul;163(7):1989-1992. doi: 10.1007/s00705-018-3802-1. Epub 2018 Mar 22.
A bipartite begomovirus was identified from a Boehmeria leiophylla plant sample exhibiting yellow mosaic symptoms collected in Nabanhe National Nature Reserve, Xishuangbanna, Yunnan, China. Five complete DNA-A and four DNA-B genome sequences were obtained by rolling-circle amplification (RCA), cloned, and sequenced. All DNA-A sequences were determined to be 2759 nucleotides in size, sharing 99.9%-100% nucleotide sequence identity with each other. The DNA-B sequences were comprised of 2673 nucleotides, sharing 98.6-100% nucleotide sequence identity with each other. Genomic organization of the begomovirus was typical of Old World bipartite begomoviruses. Sequence analysis revealed 81.84% nucleotide sequence identity to tomato leaf curl Hsinchu virus (ToLCHsV) from China for the DNA A component and 67.23% identity to the squash leaf curl China virus (SLCCNV) from India for the DNA B component. The sequence comparisons suggest that this bipartite begomovirus represents a novel species for which we propose the name "Ramie yellow mosaic virus".
从中国云南省西双版纳纳板河国家级自然保护区采集的一株表现出黄色花叶症状的苎麻植物样本中鉴定出一种双生病毒。通过滚环扩增（RCA）获得了五条完整的DNA-A和四条DNA-B基因组序列，进行克隆并测序。所有DNA-A序列大小均为2759个核苷酸，彼此之间的核苷酸序列同一性为99.9%-100%。DNA-B序列由2673个核苷酸组成，彼此之间的核苷酸序列同一性为98.6%-100%。该双生病毒的基因组结构是典型的旧世界双生病毒。序列分析显示，DNA A组分与来自中国的番茄卷叶新竹病毒（ToLCHsV）的核苷酸序列同一性为81.84%，DNA B组分与来自印度的南瓜卷叶中国病毒（SLCCNV）的同一性为67.23%。序列比较表明，这种双生病毒代表了一个新物种，我们将其命名为“苎麻黄花叶病毒”。
