Leke Walter N, Khatabi Behnam, Mignouna Djana B, Brown Judith K, Fondong Vincent N
Institute of Agricultural Research for Development (IRAD), P.O. Box 2123, Messa, Yaounde, Cameroon.
Department of Biological Sciences, Delaware State University, 1200 N. DuPont Highway, Dover, DE, 19901-2277, USA.
Arch Virol. 2016 Aug;161(8):2329-33. doi: 10.1007/s00705-016-2894-8. Epub 2016 May 25.
Here, we report the complete genome sequence of a novel bipartite begomovirus isolated from cotton plants (Gossypium raimondii, Malvaceae) exhibiting light yellow mosaic symptoms. The genome sequence was determined by Illumina DNA sequencing and confirmed by Sanger sequencing of RCA-enriched, cloned circular genomic components. The DNA-A and DNA-B components were each ~2.7 kb in size, and their genome arrangement was characteristic of other Old World bipartite begomoviruses. While the DNA-A component was most closely related to tobacco leaf curl Comoros virus (TbLCKMV) at 80 %, the DNA-B component had as its closet relative soybean chlorotic blotch virus (SbCBV) at 66 %. This previously undescribed begomovirus is herein named "cotton yellow mosaic virus" (CYMV).
在此，我们报告了一种从表现出浅黄花叶症状的棉花植株（雷蒙德氏棉，锦葵科）中分离出的新型双分体菜豆金色花叶病毒的全基因组序列。通过Illumina DNA测序确定了基因组序列，并通过对滚环扩增富集的克隆环状基因组组分进行桑格测序进行了确认。DNA-A和DNA-B组分的大小均约为2.7 kb，其基因组排列具有其他旧大陆双分体菜豆金色花叶病毒的特征。虽然DNA-A组分与烟草曲叶科摩罗病毒（TbLCKMV）的亲缘关系最为密切，相似度为80%，但DNA-B组分与其亲缘关系最近的是大豆褪绿斑点病毒（SbCBV），相似度为66%。这种此前未描述过的菜豆金色花叶病毒在此被命名为“棉花黄花叶病毒”（CYMV）。
