Kim Hyun Kyung, Lee Seung Ju, Hwang Bang-Yeon, Yoon Jong Ung, Kim Gil-Hah
Department of Plant Medicine, Chungbuk National University, Cheongju, 28644, Korea.
College of Pharmacy, Chungbuk National University, 194-21 Osong Life1-ro, Osong, Cheongju-si, Chungbuk, Korea.
Exp Appl Acarol. 2018 Apr;74(4):403-414. doi: 10.1007/s10493-018-0249-8. Epub 2018 Mar 22.
The acaricidal activity of a methanolic extract and fractions from the rhizome of Cnidium officinale against Dermanyssus gallinae adults was investigated. The C. officinale methanolic extract exhibited 100% acaricidal activity after 48 h of treatment at a dose of 4000 ppm. The acaricidal constituents of the plant were sequentially partitioned with several solvents and then purified using silica gel column chromatography and high-performance liquid chromatography. Gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy revealed (Z)-ligustilide as a constituent of C. officinale. Acaricidal activity was examined in three experimental tests (spray, fumigation and contact), with the spraying method being the most effective. The methanolic extract of C. officinale showed both contact and fumigant activities, though only fumigant activity was observed with (Z)-ligustilide. The fumigant effects of the methanolic extract and (Z)-ligustilide caused 86.5 and 62.6% mortality, respectively, of D. gallinae adults at 48 h. Among (Z)-ligustilide, acaricides (bifenthrin, cypermethrin and spinosad) and butylidenephthalide, bifenthrin displayed the highest acaricidal activity, and the activity of butylidenephthalide was 2.3-fold higher than that of (Z)-ligustilide. These results suggest that C. officinale-derived material can be used for the development of a control agent for D. gallinae.
研究了蛇床子根茎甲醇提取物及其馏分对鸡皮刺螨成虫的杀螨活性。蛇床子甲醇提取物在4000 ppm剂量处理48小时后表现出100%的杀螨活性。该植物的杀螨成分依次用几种溶剂进行分配，然后通过硅胶柱色谱和高效液相色谱进行纯化。气相色谱-质谱联用和核磁共振光谱分析表明，（Z）-藁本内酯是蛇床子的一种成分。通过喷雾、熏蒸和接触三种实验测试考察杀螨活性，其中喷雾法最为有效。蛇床子甲醇提取物表现出接触和熏蒸活性，而（Z）-藁本内酯仅表现出熏蒸活性。甲醇提取物和（Z）-藁本内酯的熏蒸作用在48小时时分别导致鸡皮刺螨成虫86.5%和62.6%的死亡率。在（Z）-藁本内酯、杀螨剂（联苯菊酯、氯氰菊酯和多杀霉素）和丁烯基苯酞中，联苯菊酯表现出最高的杀螨活性，丁烯基苯酞的活性比（Z）-藁本内酯高2.3倍。这些结果表明，蛇床子衍生材料可用于开发鸡皮刺螨防治剂。
