Yandle T, Crozier I, Nicholls G, Espiner E, Carne A, Brennan S
Biochem Biophys Res Commun. 1987 Jul 31;146(2):832-9. doi: 10.1016/0006-291x(87)90606-1.
Two atrial natriuretic peptides were purified from pooled human coronary sinus plasma by Sep-Pak extraction, immunoaffinity chromatography and reverse phase HPLC. The amino acid sequences of the two peptides were homologous with 99-126 human atrial natriuretic peptide (hANP) and 106-126 hANP, the latter being most probably linked to 99-105 ANP by the disulphide bond. The molar ratio of the peptides in plasma, as assessed by radioimmunoassay was 10:3.
通过Sep-Pak萃取、免疫亲和色谱法和反相高效液相色谱法从汇集的人冠状窦血浆中纯化出两种心房利钠肽。这两种肽的氨基酸序列与99 - 126人心房利钠肽（hANP）和106 - 126 hANP同源，后者很可能通过二硫键与99 - 105 ANP相连。通过放射免疫测定法评估，血浆中这两种肽的摩尔比为10:3。
