Lents Maicon Pereira, Barbosa Larissa Pires, Santana Ana Lúcia Almeida, Pinheiro Emmanuel Emydio Gomes, Mugabe Lopes César, Biscarde Carmo Emanuel Almeida, Kiya Cláudia Kazumi, Machado William Morais, Souza Rosileia Silva
Center for Agrarian, Environmental and Biological Sciences, Federal University of Recôncavo of Bahia, Cruz das Almas, BA, Brazil.
Center for Agrarian, Environmental and Biological Sciences, Federal University of Recôncavo of Bahia, Cruz das Almas, BA, Brazil.
Theriogenology. 2018 Jul 1;114:7-13. doi: 10.1016/j.theriogenology.2018.03.013. Epub 2018 Mar 15.
The aim of this study was to evaluate the immuno-sterilizing action of anti-gonadotrophin-releasing hormone (anti-GnRH) vaccine in goats. Eighteen male goats were randomly distributed to receive three treatments: T1 (control) - whole animals, and T2 and T3 - application of 0.5 and 1.0 mL of anti-GnRH vaccine, respectively, with six replicates and one goat per experimental unit. Vaccine was administered at 8 months of age and 30 days after the first immunization. Testicular biometry was evaluated monthly, along with seminal collections, for the physical and morphological evaluation of semen. At the time of slaughter, the testicle were collected, and fragments were measured and removed for histological evaluation. The data were evaluated for normality by the Shapiro-Wilk test, followed by appropriate statistical tests for each variable. A reduction in width and length of the right and left testicles was observed and, consequently, the scrotal circumference of the immunized animals reduced after the second vaccine application (P < 0.05). Thirty-days after the first vaccine application, there was a negative effect on seminal production and quality; and 60 days after the second application, a pronounced reduction was observed in all seminal parameters in the vaccinated animals, including azoospermia (83.33% of animals; P < 0.05). Vaccine application reduced testicular weight, seminiferous tubule diameter, and gonadosomatic and tubulosomatic index (P < 0.05), but did not influence the proportion of testicular parenchyma components (P > 0.05). Two applications of the anti-GnRH conjugate are effective for the immunological castration of goats, and the 0.5 mL dose is recommended for use in crossbred goats.
本研究的目的是评估抗促性腺激素释放激素（anti-GnRH）疫苗对山羊的免疫去势作用。18只雄性山羊被随机分为三组接受三种处理：T1（对照组）——完整动物；T2和T3——分别接种0.5 mL和1.0 mL抗GnRH疫苗，每组6个重复，每个实验单元1只山羊。疫苗在8月龄时及首次免疫后30天接种。每月评估睾丸生物测量指标，并采集精液进行精液的物理和形态学评估。屠宰时，采集睾丸，测量并取出组织块进行组织学评估。数据通过Shapiro-Wilk检验评估正态性，然后对每个变量进行适当的统计检验。观察到左右睾丸的宽度和长度减小，因此，第二次接种疫苗后，免疫动物的阴囊周长减小（P<0.05）。首次接种疫苗30天后，对精液产量和质量有负面影响；第二次接种60天后，接种疫苗的动物所有精液参数均显著降低，包括无精子症（83.33%的动物；P<0.05）。接种疫苗降低了睾丸重量、曲细精管直径、性腺体指数和曲细精管体指数（P<0.05），但不影响睾丸实质成分的比例（P>0.05）。两次接种抗GnRH偶联物对山羊进行免疫去势有效，建议0.5 mL剂量用于杂交山羊。
