Di Mattia Diana, Fondevila Dolores, Abramo Francesca, Fondati Alessandra
Veterinaria Praxis, Via Nazionale, 68, 83013, Mercogliano, Avellino, Italy.
Department of Animal Medicine and Surgery, Universitat Autònoma de Barcelona (UAB), 08193, Bellaterra, Barcelona, Spain.
Vet Dermatol. 2018 Jun;29(3):212-e76. doi: 10.1111/vde.12535. Epub 2018 Mar 25.
Head and neck ulcers in cats can arise from allergic and nonallergic disorders, including feline leishmaniosis (FeL). It is important to rule out this aetiological agent in regions that are endemic for canine leishmaniosis, because the drugs used to treat immune-mediated disorders of cats can be contraindicated in the setting of infection.
HYPOTHESIS/OBJECTIVES: The aim of this study was to evaluate the skin of cats with ulcerative dermatitis of the head or neck for evidence of Leishmania infection using combined immunohistochemistry (IHC) and Polymerase Chain Reaction (PCR). An IHC for tissue histiocytes was also utilized because leishmaniosis may provoke a histiocytic inflammatory response.
Twenty seven cats with head and/or neck ulcers.
Skin biopsy specimens were examined for the presence of Leishmania spp. by routine histopathological evaluation and IHC using a polyclonal anti-Leishmania antibody, and by quantitative PCR (qPCR). The ionized calcium-binding adapter molecule-1 (IBA-1) antibody was used to immunolocalize histiocytes. Selected history and clinical data were recorded.
All specimens showed a superficial mid-perivascular mixed inflammatory infiltrate. The presence of histiocytes was confirmed in 23 of 27 cases with the IBA-1 antibody. Immunohistochemistry and qPCR techniques confirmed the absence of Leishmania in all cases.
Leishmania did not seem to play a role in the pathogenesis of feline ulcerative dermatitis of the head and neck in the subjects studied, despite a lifestyle potentially associated with infection. Histiocytic infiltration of tissue is not a specific marker for Leishmania infection in this population.
猫的头颈部溃疡可由过敏性和非过敏性疾病引起，包括猫利什曼病（FeL）。在犬利什曼病流行地区，排除这种病原体很重要，因为用于治疗猫免疫介导性疾病的药物在感染情况下可能是禁忌的。
假设/目的：本研究的目的是使用联合免疫组织化学（IHC）和聚合酶链反应（PCR）评估患有头颈部溃疡性皮肤病的猫的皮肤，以寻找利什曼原虫感染的证据。还利用了针对组织组织细胞的免疫组织化学，因为利什曼病可能引发组织细胞性炎症反应。
27只患有头颈部溃疡的猫。
通过常规组织病理学评估和使用多克隆抗利什曼原虫抗体的免疫组织化学以及定量PCR（qPCR），检查皮肤活检标本中是否存在利什曼原虫属。使用离子钙结合衔接分子-1（IBA-1）抗体对组织细胞进行免疫定位。记录选定的病史和临床数据。
所有标本均显示浅层血管周围混合性炎症浸润。27例中有23例使用IBA-1抗体证实存在组织细胞。免疫组织化学和qPCR技术证实所有病例均未发现利什曼原虫。
在所研究的对象中，尽管生活方式可能与感染有关，但利什曼原虫似乎在猫头颈部溃疡性皮肤病的发病机制中不起作用。组织细胞浸润在该人群中不是利什曼原虫感染的特异性标志物。
