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腺病毒早期区域1A产物对原癌基因c-fos和c-myc而非c-Ha-ras的启动子反式激活作用。

Promoter trans-activation of protooncogenes c-fos and c-myc, but not c-Ha-ras, by products of adenovirus early region 1A.

作者信息

Sassone-Corsi P, Borrelli E

出版信息

Proc Natl Acad Sci U S A. 1987 Sep;84(18):6430-3. doi: 10.1073/pnas.84.18.6430.

DOI:10.1073/pnas.84.18.6430
PMID:2957694
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC299090/
Abstract

The E1A (early region 1A) oncogene products of adenovirus type 2 trans-activate the other early viral transcription units, as well as some cellular promoters. Using a short-term cotransfection assay in murine NIH 3T3 fibroblasts, we show that c-fos and c-myc promoter activities are stimulated by the E1A proteins, whereas c-Ha-ras transcription is not affected. The product of E1A 13S mRNA is responsible for the trans-activation, whereas the 12S mRNA product has no effect. Analysis of the c-fos promoter sequences required for the E1A stimulation shows that responsive sequences are located between positions -402 and -240 upstream of the transcription initiation site. This same region also contains the c-fos serum-responsive element. Furthermore, transcription of the endogenous c-fos gene in HeLa cells is increased after E1A transfection.

摘要

2型腺病毒的E1A（早期区域1A）癌基因产物可反式激活其他早期病毒转录单位以及一些细胞启动子。通过在小鼠NIH 3T3成纤维细胞中进行短期共转染试验，我们发现E1A蛋白可刺激c-fos和c-myc启动子活性，而c-Ha-ras转录不受影响。E1A 13S mRNA的产物负责反式激活，而12S mRNA产物则无此作用。对E1A刺激所需的c-fos启动子序列进行分析表明，反应序列位于转录起始位点上游-402至-240位之间。该区域还包含c-fos血清反应元件。此外，在E1A转染后，HeLa细胞中内源性c-fos基因的转录增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b4/299090/476368e80a2f/pnas00333-0116-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b4/299090/42f18b1612a1/pnas00333-0115-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b4/299090/c6cb8f1741fa/pnas00333-0116-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b4/299090/476368e80a2f/pnas00333-0116-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b4/299090/42f18b1612a1/pnas00333-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b4/299090/534081f15b33/pnas00333-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b4/299090/c6cb8f1741fa/pnas00333-0116-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54b4/299090/476368e80a2f/pnas00333-0116-c.jpg

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本文引用的文献

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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
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Cyclic AMP induction of early adenovirus promoters involves sequences required for E1A trans-activation.环磷酸腺苷（cAMP）对腺病毒早期启动子的诱导涉及E1A反式激活所需的序列。
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c-fos promoter trans-activation by the tax1 protein of human T-cell leukemia virus type I.人I型T细胞白血病病毒tax1蛋白对c-fos启动子的反式激活作用
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Bovine leukemia virus trans-activator p38tax activates heterologous promoters with a common sequence known as a cAMP-responsive element or the binding site of a cellular transcription factor ATF.牛白血病病毒反式激活因子p38tax通过一个被称为环磷酸腺苷反应元件或细胞转录因子ATF结合位点的共同序列激活异源启动子。
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E1A-dependent trans-activation of the human MYC promoter is mediated by the E2F factor.E1A 依赖的人 MYC 启动子反式激活由 E2F 因子介导。
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HeLa cell beta-tubulin gene transcription is stimulated by adenovirus 5 in parallel with viral early genes by an E1a-dependent mechanism.人乳头瘤病毒（HeLa）细胞的β-微管蛋白基因转录受5型腺病毒刺激，通过E1a依赖机制与病毒早期基因同时被激活。
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