蒺藜苜蓿脂肪酸去饱和酶基因的全基因组鉴定和表达分析。

Genome-wide identification and expression analysis of the fatty acid desaturase genes in Medicago truncatula.

机构信息

State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China.

出版信息

Biochem Biophys Res Commun. 2018 May 5;499(2):361-367. doi: 10.1016/j.bbrc.2018.03.165. Epub 2018 Mar 27.

DOI:10.1016/j.bbrc.2018.03.165

PMID:29577909

Abstract

Fatty acid desaturases (FADs) are of great importance and play critical roles in regulating plant fatty acid (FA) compositions. But to date, no reports about characterization of the FAD genes have been reported in the model dicotyledonous grass species Medicago truncatula. In this study, using database searches, 20 full-length FAD genes were identified in M. truncatula. These FAD genes were unevenly distributed on six chromosomes except the chromosome 6 and 8. Phylogenetic analysis showed the FAD genes in M. truncatula were clustered into six subfamilies and had similar exon number and intron phase in the same subfamily. Moreover, expression analysis based on qRT-PCR indicated these FAD genes were extensively involved in cold and heat responses. This study would provide an important foundation for future cloning and functional studies of FAD genes in M. truncatula and other related legume species.

摘要

脂肪酸去饱和酶（FADs）在调节植物脂肪酸（FA）组成方面具有重要意义，发挥着关键作用。但迄今为止，在模式双子叶草本植物百脉根（Medicago truncatula）中，尚未有关于 FAD 基因特征的报道。在本研究中，通过数据库搜索，鉴定了 20 个全长 FAD 基因在百脉根中的存在。这些 FAD 基因不均匀地分布在除了第 6 和 8 号染色体之外的其余六条染色体上。系统发生分析表明，百脉根中的 FAD 基因聚类为六个亚家族，并且在同一亚家族中具有相似的外显子数量和内含子相位。此外，基于 qRT-PCR 的表达分析表明，这些 FAD 基因广泛参与冷和热反应。本研究为进一步克隆和功能研究百脉根和其他相关豆科物种的 FAD 基因提供了重要基础。