Giusto Gessica, Beretta Giangiacomo, Vercelli Cristina, Valle Emanuela, Iussich Selina, Borghi Roberta, Odetti Patrizio, Monacelli Fiammetta, Tramuta Clara, Grego Elena, Nebbia Patrizia, Robino Patrizia, Odore Rosangela, Gandini Marco
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2-5, 10095 Grugliasco (TO), Italy.
Department of Pharmaceutical Sciences DISFARM, University of Milan, Via Mangiagalli 25, 20133 Milano (MI), Italy.
Biomed Mater Eng. 2018;29(3):347-356. doi: 10.3233/BME-181730.
Novel pectin-honey hydrogels have been developed and characterized as medical device. Ideally, a wound dressing should maintain optimal fluid affinity, permit moisture evaporation, protect the wound from microbes, and have shape-conformability, biocompatibility, and antibacterial activity.
A novel, simple and fast method to produce pectin-honey wound dressings is described.
The properties of these pectin-honey hydrogels were investigated, including swelling ability, water vapour transmission rate, hydrogen peroxide production, methylglyoxal content and antibacterial activity. Biocompatibility was assessed by proliferation assays using cultured fibroblast cells and by in vivo study with subcutaneous and intraperitoneal implantation in rats.
Hydrogel showed a good water vapour transmission rate, fluid uptake and were not cytotoxic for fibroblasts. The hydrogel demonstrated good antibacterial activity toward clinically relevant pathogens, including S. aureus and E. coli. Biocompatibility was confirmed by the measurement of plasma levels of interleukin (IL)1 beta, IL-6, tumour necrosis factor (TNF) alpha, and prostaglandin (PG)E2. No histological changes were observed.
The presence of a natural active component, conformability, and complete resorbability are the main characteristics of this new biocompatible biomaterial that is well tolerated by the body, possibly improves healing, may be used for surgical complications prevention, with a simple and inexpensive production process.
新型果胶-蜂蜜水凝胶已被开发并作为医疗器械进行了表征。理想情况下，伤口敷料应保持最佳的液体亲和力，允许水分蒸发，保护伤口免受微生物侵害，并具有形状适应性、生物相容性和抗菌活性。
描述一种生产果胶-蜂蜜伤口敷料的新颖、简单且快速的方法。
研究了这些果胶-蜂蜜水凝胶的特性，包括膨胀能力、水蒸气透过率、过氧化氢生成量、甲基乙二醛含量和抗菌活性。通过使用培养的成纤维细胞进行增殖测定以及在大鼠体内进行皮下和腹腔内植入的体内研究来评估生物相容性。
水凝胶显示出良好的水蒸气透过率、液体吸收能力，且对成纤维细胞无细胞毒性。该水凝胶对包括金黄色葡萄球菌和大肠杆菌在内的临床相关病原体表现出良好的抗菌活性。通过测量白细胞介素（IL）1β、IL-6、肿瘤坏死因子（TNF）α和前列腺素（PG）E2的血浆水平证实了生物相容性。未观察到组织学变化。
这种新型生物相容性生物材料的主要特征是存在天然活性成分、适应性和完全可吸收性，它能被身体很好地耐受，可能改善愈合情况，可用于预防手术并发症，且生产过程简单、成本低廉。
