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Toxins (Basel). 2020 Sep 15;12(9):595. doi: 10.3390/toxins12090595.
toxins are emerging mycotoxins, candidates for regulation by European Authorities. Therefore, highly sensitive, confirmatory, and reliable analytical methodologies are required for their monitoring in food. In that context, an isotope dilution LC-MS/MS method was developed for the analysis of five toxins (Altenuene, Alternariol, Alternariol monomethylether, Tentoxin, and Tenuazonic Acid) in a broad range of commodities including cereals and cereal-based products, tomato-based products, tree nuts, vegetable oils, dried fruits, cocoa, green coffee, spices, herbs, and tea. Validation data collected in two different laboratories demonstrated the robustness of the method. Underestimation of Tenuazonic Acid level in dry samples such as cereals was reported when inappropriate extraction solvent mixtures were used as currently done in several published methodologies. An investigation survey performed on 216 food items evidenced large variations of toxins levels, in line with data reported in the last EFSA safety assessment. The analysis of 78 green coffee samples collected from 21 producing countries demonstrated that coffee is a negligible source of exposure to toxins. Its wide scope of application, adequate sample throughput, and high sensitivity make this method fit for purpose for the regular monitoring of toxins in foods.
真菌毒素是新兴的霉菌毒素，也是欧洲当局监管的候选物。因此，需要高度敏感、确证和可靠的分析方法来监测食品中的真菌毒素。在这种情况下，开发了一种同位素稀释 LC-MS/MS 方法，用于分析包括谷物和谷物制品、番茄制品、坚果、植物油、干果、可可、未烘焙咖啡、香料、草药和茶在内的广泛商品中的五种真菌毒素（altenuene、 alternariol、alternariol monomethylether、tentoxin 和 tenuazonic acid）。在两个不同的实验室收集的验证数据证明了该方法的稳健性。当使用目前在几个已发表的方法中使用的不合适的提取溶剂混合物时，报告了谷物等干燥样品中 tenuazonic acid 水平的低估。对 216 种食品进行的调查表明，真菌毒素的水平存在很大差异，与 EFSA 最近的安全评估中报告的数据一致。对从 21 个生产国收集的 78 个未烘焙咖啡样品的分析表明，咖啡是接触真菌毒素的可忽略来源。其广泛的应用范围、足够的样品通量和高灵敏度使该方法适合用于定期监测食品中的真菌毒素。
