Cole Jessica K, Morton Beau R, Cardamone Hayley C, Lake Hannah R R, Dohnalkova Alice C, Kim Young-Mo, Kyle Jennifer E, Maezato Yukari, Dana Karl L, Metz Thomas O, Romine Margaret F, Nelson William C, Lindemann Stephen R
Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA.
Scientific and Computing Operations, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
Int J Syst Evol Microbiol. 2018 May;68(5):1591-1598. doi: 10.1099/ijsem.0.002715. Epub 2018 Mar 26.
A halophilic bacterial strain, HL-109, was isolated from the unicyanobacterial consortium UCC-O, which was obtained from the photosynthetic mat of Hot Lake (Washington, USA). A polyphasic approach using phenotypic, genotypic and chemotaxonomic data was used to classify the strain within the order Rhizobiales. The organism stained Gram-negative and was a moderate thermophile with a growth optimum of 45 °C. It was obligately aerobic, heterotrophic and halophilic, growing in both NaCl and MgSO4 brines. The novel isolate had a polymorphic cellular morphology of short rods with occasional branching, and cells were monotrichous. The major fatty acids detected were C18 : 1, C18 : 0, C16 : 0 and C18 : cyc. Phylogenetic analysis of the 16S rRNA gene placed the strain in the order Rhizobiales and it shared 94 % identity with the type strain of its nearest relative, Salinarimonas ramus. Morphological, chemotaxonomic and phylogenetic results did not affiliate the novel organism with any of the families in the Rhizobiales; therefore, HL-109 is representative of a new lineage, for which the name Salinivirga fredricksonii gen. nov., sp. nov. is proposed, with the type strain HL-109 (=JCM 31876=DSM 102886). In addition, examination of the phylogenetics of strain HL-109 and its nearest relatives, Salinarimonas ramus and Salinarimonasrosea, demonstrates that these halophiles form a clade distinct from the described families of the Rhizobiales. We further propose the establishment of a new family, Salinarimonadaceae fam. nov., to accommodate the genera Salinivirga and Salinarimonas (the type genus of the family).
从单蓝细菌聚生体UCC - O中分离出一株嗜盐细菌HL - 109，该聚生体取自美国华盛顿州热湖的光合垫。采用多相分类法，利用表型、基因型和化学分类学数据对该菌株进行分类，确定其属于根瘤菌目。该菌革兰氏染色阴性，为中度嗜热菌，最适生长温度为45°C。它是专性需氧、异养和嗜盐的，能在NaCl和MgSO4盐水中生长。该新分离菌株具有多态性细胞形态，为短杆状，偶尔有分支，细胞为单端鞭毛。检测到的主要脂肪酸为C18 : 1、C18 : 0、C16 : 0和C18 : cyc。对16S rRNA基因的系统发育分析表明，该菌株属于根瘤菌目，与其最接近的亲缘种盐沼单胞菌（Salinarimonas ramus）的模式菌株有94%的序列相似性。形态学、化学分类学和系统发育学结果表明，该新菌株不属于根瘤菌目中的任何一个科；因此，HL - 109代表了一个新的谱系，为此提出新属名盐沼栖病毒菌属（Salinivirga）、新种名弗雷德里克森盐沼栖病毒菌（Salinivirga fredricksonii），模式菌株为HL - 109（=JCM 31876=DSM 102886）。此外，对菌株HL - 109及其最接近的亲缘种盐沼单胞菌和玫瑰盐沼单胞菌（Salinarimonasrosea）的系统发育研究表明，这些嗜盐菌形成了一个与根瘤菌目中已描述的科不同的进化枝。我们进一步提议建立一个新科，即盐沼单胞菌科（Salinarimonadaceae），以容纳盐沼栖病毒菌属和盐沼单胞菌属（该科的模式属）。
