Waschburger Edgar, Kulcheski Franceli Rodrigues, Veto Nicole Moreira, Margis Rogerio, Margis-Pinheiro Marcia, Turchetto-Zolet Andreia Carina
Graduação em Biotecnologia, Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
Departamento de Biologia Celular, Embriologia e Genética, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.
Genet Mol Biol. 2018;41(1 suppl 1):355-370. doi: 10.1590/1678-4685-GMB-2017-0076. Epub 2018 Mar 19.
sn-Glycerol-3-phosphate 1-O-acyltransferase (GPAT) is an important enzyme that catalyzes the transfer of an acyl group from acyl-CoA or acyl-ACP to the sn-1 or sn-2 position of sn-glycerol-3-phosphate (G3P) to generate lysophosphatidic acids (LPAs). The functional studies of GPAT in plants demonstrated its importance in controlling storage and membrane lipid. Identifying genes encoding GPAT in a variety of plant species is crucial to understand their involvement in different metabolic pathways and physiological functions. Here, we performed genome-wide and evolutionary analyses of GPATs in plants. GPAT genes were identified in all algae and plants studied. The phylogenetic analysis showed that these genes group into three main clades. While clades I (GPAT9) and II (soluble GPAT) include GPATs from algae and plants, clade III (GPAT1-8) includes GPATs specific from plants that are involved in the biosynthesis of cutin or suberin. Gene organization and the expression pattern of GPATs in plants corroborate with clade formation in the phylogeny, suggesting that the evolutionary patterns is reflected in their functionality. Overall, our results provide important insights into the evolution of the plant GPATs and allowed us to explore the evolutionary mechanism underlying the functional diversification among these genes.
sn-甘油-3-磷酸1-O-酰基转移酶（GPAT）是一种重要的酶，它催化酰基从酰基辅酶A或酰基载体蛋白转移到sn-甘油-3-磷酸（G3P）的sn-1或sn-2位，生成溶血磷脂酸（LPA）。植物中GPAT的功能研究表明其在控制储存和膜脂方面的重要性。在多种植物物种中鉴定编码GPAT的基因对于理解它们参与不同的代谢途径和生理功能至关重要。在这里，我们对植物中的GPAT进行了全基因组和进化分析。在所有研究的藻类和植物中都鉴定出了GPAT基因。系统发育分析表明，这些基因分为三个主要分支。虽然分支I（GPAT9）和分支II（可溶性GPAT）包括来自藻类和植物的GPAT，但分支III（GPAT1-8）包括植物特有的参与角质或木栓质生物合成的GPAT。植物中GPAT的基因组织和表达模式与系统发育中的分支形成相一致，表明进化模式反映在它们的功能上。总体而言，我们的结果为植物GPAT的进化提供了重要见解，并使我们能够探索这些基因功能多样化背后的进化机制。
