Tollervey D
Unité des Applications du Génie Génétique Institut Pasteur, Paris, France.
J Mol Biol. 1987 Jul 20;196(2):355-61. doi: 10.1016/0022-2836(87)90696-6.
The complexity of the trimethylguanosine-capped, small nuclear RNA (snRNA) populations in a number of organisms has been examined using immunoprecipitation and two-dimensional gels. From the fungi Aspergillus nidulans and Schizosaccharomyces pombe, over 30 major snRNAs can be resolved. The most abundant of these correspond to the putative analogues of vertebrate U1, U2, U4 and U5, which have been reported to be precipitated by anti-Sm antibodies, but other snRNAs are little less abundant than the major Sm-precipitable species. A similarly high level of complexity of snRNAs is detected in pea plants. In Candida albicans, the snRNAs are somewhat less numerous (about 22 major species) and are substantially less abundant than those of the above fungi, features shared with another budding yeast, Saccharomyces cerevisiae. Ten species of human snRNA have been reported; on two-dimensional gels, a number of additional snRNAs can be resolved from human cells. Each fungus, as well as pea plants, contains snRNAs substantially larger than any reported from vertebrates or detected in the human RNA used here. It appears that many eukaryotes contain substantially more species of snRNA than was previously believed.
利用免疫沉淀法和二维凝胶电泳技术，对多种生物体中三甲基鸟苷帽化的小核RNA（snRNA）群体的复杂性进行了研究。在构巢曲霉和粟酒裂殖酵母这两种真菌中，可以分辨出30多种主要的snRNA。其中最丰富的对应于脊椎动物U1、U2、U4和U5的假定类似物，据报道这些类似物可被抗Sm抗体沉淀，但其他snRNA的丰度略低于主要的可被Sm沉淀的物种。在豌豆植株中也检测到了类似的高水平snRNA复杂性。在白色念珠菌中，snRNA的种类略少（约22种主要种类），且丰度远低于上述真菌，这一特征与另一种芽殖酵母酿酒酵母相同。据报道，人类有10种snRNA；在二维凝胶电泳上，可以从人类细胞中分辨出许多其他的snRNA。每种真菌以及豌豆植株都含有比脊椎动物报道的或此处所用人类RNA中检测到的任何snRNA都大得多的snRNA。看来许多真核生物含有的snRNA种类比以前认为的要多得多。
