Soybean Research Institute, National Center for Soybean Improvement, Key Laboratory of Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production Nanjing Agricultural University, Nanjing 210095, China, Nanjing Agricultural University, Nanjing, 210095, China.
BMC Genomics. 2018 Mar 27;19(1):226. doi: 10.1186/s12864-018-4582-4.
Plant height (PH) is an important agronomic trait and is closely related to yield in soybean [Glycine max (L.) Merr.]. Previous studies have identified many QTLs for PH. Due to the complex genetic background of PH in soybean, there are few reports on its fine mapping.
In this study, we used a mapping population derived from a cross between a chromosome segment substitution line CSSL3228 (donor N24852 (G. Soja), a receptor NN1138-2 (G. max)) and NN1138-2 to fine map a wild soybean allele of greater PH by QTL-seq and linkage mapping. We identified a QTL for PH in a 1.73 Mb region on soybean chromosome 13 through QTL-seq, which was confirmed by SSR marker-based classical QTL mapping in the mapping population. The linkage analysis showed that the QTLs of PH were located between the SSR markers BARCSOYSSR_13_1417 and BARCSOYSSR_13_1421 on chromosome 13, and the physical distance was 69.3 kb. RT-PCR and sequence analysis of possible candidate genes showed that Glyma.13 g249400 revealed significantly higher expression in higher PH genotypes, and the gene existed 6 differences in the amino acids encoding between the two parents.
Data presented here provide support for Glyma.13 g249400 as a possible candidate genes for higher PH in wild soybean line N24852.
株高（PH）是一个重要的农艺性状，与大豆产量密切相关。先前的研究已经确定了许多与 PH 相关的 QTL。由于大豆 PH 的遗传背景复杂，关于其精细定位的报道很少。
在这项研究中，我们利用一个来自染色体片段代换系 CSSL3228（供体 N24852（G. Soja），受体 NN1138-2（G. max））与 NN1138-2 杂交的作图群体，通过 QTL-seq 和连锁作图精细定位了一个更大 PH 的野生大豆等位基因。我们通过 QTL-seq 在大豆第 13 号染色体上一个 1.73 Mb 的区域鉴定到一个 PH 的 QTL，该 QTL在作图群体中通过 SSR 标记的经典 QTL 作图得到了验证。连锁分析表明，PH 的 QTL 位于第 13 号染色体上的 SSR 标记 BARCSOYSSR_13_1417 和 BARCSOYSSR_13_1421 之间，物理距离为 69.3 kb。可能的候选基因的 RT-PCR 和序列分析表明，Glyma.13g249400 在高 PH 基因型中表现出明显更高的表达，并且该基因在两个亲本之间的氨基酸编码上存在 6 个差异。
本文提供的数据支持 Glyma.13g249400 作为野生大豆品系 N24852 中高 PH 的一个可能的候选基因。
