Halkai Kiran Rahul, Mudda Jayashree A, Shivanna Vasundhara, Rathod Vandana, Halkai Rahul
Department of Conservative Dentistry and Endodontics, HKE's SN Institute of Dental Sciences and Research, Kalaburagi, India.
Department of Periodontics, HKE's SN Institute of Dental Sciences and Research, Kalaburagi, India.
Contemp Clin Dent. 2018 Jan-Mar;9(1):45-48. doi: 10.4103/ccd.ccd_703_17.
The main objective of endodontic therapy is complete elimination and prevention of bacteria from the root canal system; however, it is difficult due to anatomical ramifications of root canal system and growing resistant microbes to available disinfectants. Therefore, to overcome this problem, newer antimicrobial agents have to be developed.
The aim of the study was to evaluate the antibacterial efficacy of fungal-derived biosynthesized silver nanoparticles (AgNPs) against .
Freshly prepared silver nanoparticles using the endophytic fungi , characterized by different techniques were used to evaluate the antibacterial efficacy against by agar well diffusion method measuring the zone of inhibition using different concentrations of nanoparticles (AgNPs) (A [20 μl], B [40 μl], C [60 μl], D [80 μl], and E [100 μl]), (0.2% chlorhexidine [CHX]), G (2% CHX), H (ampicillin), and I (distilled water) were used as control groups. Statistical Analysis Used: One-way ANOVA followed by Tukey multiple comparison test was done.
AgNPs (100 ml) showed highest zone of inhibition 19.5 mm against . CHX (0.2%) 14.52 mm, CHX (2%) 20.02 mm, and ampicillin showed highest mean zone of inhibition 20.5 mm and distilled water showed no zone of inhibition. Results indicate no significant difference between E (100 μl), G (2% CHX), and H (ampicillin) ( < 0.0001).
Biosynthesized AgNPs exhibit efficient antibacterial activity against and therefore can be used as root canal irrigant or intracanal medicament for root canal disinfection.
根管治疗的主要目标是彻底清除并预防根管系统中的细菌；然而，由于根管系统的解剖分支以及现有消毒剂对不断产生耐药性的微生物，这一目标难以实现。因此，为克服这一问题，必须研发新型抗菌剂。
本研究旨在评估真菌生物合成的银纳米颗粒（AgNPs）对……的抗菌效果。
使用内生真菌新鲜制备的银纳米颗粒，通过不同技术进行表征，采用琼脂扩散法，使用不同浓度的纳米颗粒（AgNPs）（A组[20微升]、B组[40微升]、C组[60微升]、D组[80微升]和E组[100微升]）评估对……的抗菌效果，将0.2%氯己定（CHX）、G组（2%CHX）、H组（氨苄青霉素）和I组（蒸馏水）作为对照组。统计分析方法：采用单因素方差分析，随后进行Tukey多重比较检验。
AgNPs（100微升）对……显示出最高抑菌圈，为19.5毫米。0.2%CHX的抑菌圈为14.52毫米，2%CHX的抑菌圈为20.02毫米，氨苄青霉素显示出最高平均抑菌圈，为20.5毫米，蒸馏水无抑菌圈。结果表明E组（100微升）、G组（2%CHX）和H组（氨苄青霉素）之间无显著差异（P<0.0001）。
生物合成的AgNPs对……表现出高效抗菌活性，因此可作为根管冲洗剂或根管内药物用于根管消毒。
