BACKGROUND

The main objective of endodontic therapy is complete elimination and prevention of bacteria from the root canal system; however, it is difficult due to anatomical ramifications of root canal system and growing resistant microbes to available disinfectants. Therefore, to overcome this problem, newer antimicrobial agents have to be developed.

AIMS

The aim of the study was to evaluate the antibacterial efficacy of fungal-derived biosynthesized silver nanoparticles (AgNPs) against .

MATERIALS AND METHODS

Freshly prepared silver nanoparticles using the endophytic fungi , characterized by different techniques were used to evaluate the antibacterial efficacy against by agar well diffusion method measuring the zone of inhibition using different concentrations of nanoparticles (AgNPs) (A [20 μl], B [40 μl], C [60 μl], D [80 μl], and E [100 μl]), (0.2% chlorhexidine [CHX]), G (2% CHX), H (ampicillin), and I (distilled water) were used as control groups. Statistical Analysis Used: One-way ANOVA followed by Tukey multiple comparison test was done.

RESULTS

AgNPs (100 ml) showed highest zone of inhibition 19.5 mm against . CHX (0.2%) 14.52 mm, CHX (2%) 20.02 mm, and ampicillin showed highest mean zone of inhibition 20.5 mm and distilled water showed no zone of inhibition. Results indicate no significant difference between E (100 μl), G (2% CHX), and H (ampicillin) ( < 0.0001).

CONCLUSIONS

Biosynthesized AgNPs exhibit efficient antibacterial activity against and therefore can be used as root canal irrigant or intracanal medicament for root canal disinfection.