Guruswamy Pandian Anu Priya, Ramachandran Anil Kumar, Kodaganallur Pitchumani Priyanka, Mathai Blessy, Thomas Davis C
Endodontics, Private Practice, Los Angeles, USA.
Endodontics, Ragas Dental College and Hospital, Chennai, IND.
Cureus. 2024 Dec 9;16(12):e75421. doi: 10.7759/cureus.75421. eCollection 2024 Dec.
Introduction Complex interactions between cariogenic bacteria and host factors modulate dental caries. , a gram-positive facultative anaerobe plays a prominent role in the initiation of caries. The ability of to adhere to salivary enamel pellicle results in an acidic local habitat for the organism. This leads to demineralization of the tooth and penetration of bacteria into the pulp leading to endodontic infections. an opportunistic pathogenis a gram-positive, facultative anaerobe implicated in secondary endodontic infections. This study aimed to evaluate the anti-bacterial efficacy of zirconium oxide nanoparticles (ZrO NPs) against and Materials and methods Standard and strains were subcultured at specific temperatures for 24 hours. was subcultured onto blood agar and colonies of were cultured on nutrient agar. The strains were tested for their sensitivity to ZrO NPs at various dilutions. The standard methods determined the minimum concentration of ZrO NPs to inhibit 99.9% growth of and . Results The zones of inhibition were compared with gentamicin as a control. ZrO NPs exhibited clear zones of inhibition of 12 mm and 15 mm at 100 mg/mL concentrations against and in the agar wells, respectively. Conclusion The present study concluded that ZrO NPs have potential anti-bacterial activity against both and .
引言 致龋菌与宿主因素之间的复杂相互作用会影响龋齿的发生。变形链球菌，一种革兰氏阳性兼性厌氧菌，在龋齿的起始过程中起着重要作用。变形链球菌粘附于唾液釉质 pellicle 的能力会为该生物体营造一个酸性局部环境。这会导致牙齿脱矿，细菌侵入牙髓，进而引发牙髓感染。粪肠球菌，一种机会致病菌，是一种与继发性牙髓感染有关的革兰氏阳性兼性厌氧菌。本研究旨在评估氧化锆纳米颗粒（ZrO NPs）对变形链球菌和粪肠球菌的抗菌效果。材料与方法 将标准变形链球菌和粪肠球菌菌株在特定温度下传代培养24小时。将变形链球菌接种到血琼脂上，将粪肠球菌菌落接种到营养琼脂上。测试这些菌株在不同稀释度下对ZrO NPs的敏感性。采用标准方法确定抑制变形链球菌和粪肠球菌99.9%生长的ZrO NPs的最低浓度。结果 将抑菌圈与作为对照的庆大霉素进行比较。在琼脂孔中，ZrO NPs在100 mg/mL浓度下对变形链球菌和粪肠球菌分别表现出12 mm和15 mm的明显抑菌圈。结论 本研究得出结论，ZrO NPs对变形链球菌和粪肠球菌均具有潜在的抗菌活性。
