Garcia-Prat Marina, Vila-Pijoan Gemma, Martos Gutierrez Susana, Gala Yerga Guadalupe, García Guantes Esther, Martínez-Gallo Mónica, Martín-Nalda Andrea, Soler-Palacín Pere, Hernández-González Manuel
Pediatric Infectious Diseases and Immunodeficiencies Unit (UPIIP), Hospital Universitari Vall d'Hebron (HUVH), Vall d'Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona (UAB), Barcelona, Spain.
Jeffrey Model Foundation Excellence Center, Barcelona, Spain.
J Clin Lab Anal. 2018 Jul;32(6):e22420. doi: 10.1002/jcla.22420. Epub 2018 Mar 30.
Measurement of immunoglobulins and complement proteins are frontline tests used in the assessment of immune system integrity, and reference values can vary with age. Their measurement provides an insight into the function of the innate and adaptive immune systems.
We generated pediatric reference ranges for IgG, IgA, IgM, IgD, the IgG and IgA subclasses, and C3 and C4 using the Optilite™ turbidimetric analyzer.
The concentrations of IgG, IgA, and IgD showed an increase with age, as expected, while IgM remained stable between the age groups. For the IgG subclasses, no significant differences were observed in IgG1 or IgG3, while IgG2 and IgG4 concentrations increased steadily with age. The concentration of IgG2 plateaued at 15-18 years, while IgG4 plateaued at 10-14 years. The trend of concentrations across all groups was IgG1 > IgG2 > IgG3 > IgG4. For both IgA1 and IgA2, concentrations increased significantly with age, plateauing at 15-18 years. The median IgA1 concentration was greater than IgA2 across all groups. There was a good correlation between the total IgG or IgA concentration and summation of their subclasses (R = 0.89, P < .0001, slope y = 0.98x + 14.51 mg/dL and R = 0.91, P < .0001, slope y = 1.35x-3.28 mg/dL, respectively). The concentration of C3 and C4 remained stable across the groups, with no significant differences observed.
We have generated age-specific reference ranges in healthy children for C3, C4, IgG, IgA, IgM, IgD and the IgG and IgA subclasses using the Optilite turbidimetric analyzer. These ranges will help identify individuals with abnormal concentrations, thus will aid in the diagnosis of both primary and secondary immunological disorders.
免疫球蛋白和补体蛋白的检测是评估免疫系统完整性的一线检测方法，其参考值会因年龄而异。对它们的检测有助于深入了解先天性和适应性免疫系统的功能。
我们使用Optilite™比浊分析仪生成了儿童IgG、IgA、IgM、IgD、IgG和IgA亚类以及C3和C4的参考范围。
正如预期的那样，IgG、IgA和IgD的浓度随年龄增加，而IgM在各年龄组之间保持稳定。对于IgG亚类，IgG1或IgG3未观察到显著差异，而IgG2和IgG4的浓度随年龄稳步增加。IgG2的浓度在15 - 18岁时趋于平稳，而IgG4的浓度在10 - 14岁时趋于平稳。所有组的浓度趋势为IgG1 > IgG2 > IgG3 > IgG4。对于IgA1和IgA2，浓度均随年龄显著增加，在15 - 18岁时趋于平稳。所有组中IgA1的中位数浓度均高于IgA2。总IgG或IgA浓度与其亚类总和之间具有良好的相关性（R = 0.89，P <.0001，斜率y = 0.98x + 14.51mg/dL；以及R = 0.91，P <.0001，斜率y = 1.35x - 3.28mg/dL）。C3和C4的浓度在各组中保持稳定，未观察到显著差异。
我们使用Optilite比浊分析仪生成了健康儿童C3、C4、IgG、IgA、IgM、IgD以及IgG和IgA亚类的年龄特异性参考范围。这些范围将有助于识别浓度异常的个体，从而有助于原发性和继发性免疫疾病的诊断。
