Department of Agriculture and Food Science, Faculty of Agriculture, University of Mauritius, Réduit, Mauritius.
Department of Health Science, Faculty of Science, University of Mauritius, Réduit, Mauritius.
World J Microbiol Biotechnol. 2018 Apr 2;34(4):59. doi: 10.1007/s11274-018-2442-7.
Mangroves are unique but endangered coastal ecosystems that play a vital role in the tropical and subtropical environments. Mauritius has two species of mangroves, Bruguiera gymnorrhiza (L.) Lam. and Rhizophora mucronata Lam., growing along its coast. The mangrove rhizosphere harbours a diverse microbial community and the use of RNA-sequencing can reveal both the taxonomic composition and active biochemical functions of the complex microbial community. Metatranscriptomic study was carried out by comparing the microbial community of rhizosphere microbiomes sediments from the two mangroves species. The study also included a comparison between a natural and a man grown mangrove microbiome. Overall, samples showed predominance by Proteobacteria, Bacteroidetes and Firmicutes, with high abundance of sulphate reducers, nitrogen reducers and methanogens. Significant difference was, however, noted at both taxonomic and functional levels among the mangroves species. The data also indicate that the microbial core involved in methane, nitrogen, and sulphur metabolism consisted mainly of Burkholderiaceae, Planctomycetaceae, Rhodobacteraceae, and Desulfobacteraceae. Also, genes encoding enzymes involved in carbon cycling, the metabolism of nitrogen, methane and sulphur were dominant in the rhizosphere of the natural mangrove ecosystem. To our knowledge, this is a first metatranscriptomic study on the microbiome of mangroves in the Mauritius, and our results provide the first insights in the range of functions and microbial diversity of the local mangrove species.
红树林是独特但濒危的沿海生态系统，在热带和亚热带环境中发挥着至关重要的作用。毛里求斯有两种红树林，分别是木榄（Bruguiera gymnorrhiza (L.) Lam.）和角果木（Rhizophora mucronata Lam.），生长在其沿海地区。红树林根际蕴藏着多样的微生物群落，而使用 RNA 测序可以揭示复杂微生物群落的分类组成和活跃的生化功能。通过比较两种红树林物种的根际微生物组沉积物中的微生物群落，进行了宏转录组学研究。该研究还包括对天然和人工种植的红树林微生物组进行了比较。总体而言，样本显示出以变形菌门、拟杆菌门和厚壁菌门为主导，硫酸盐还原菌、氮还原菌和产甲烷菌的丰度很高。然而，在两个红树林物种之间，在分类和功能水平上都观察到了显著的差异。该数据还表明，参与甲烷、氮和硫代谢的微生物核心主要由伯克霍尔德氏菌科、浮霉菌科、红杆菌科和脱硫杆菌科组成。此外，参与碳循环、氮代谢、甲烷和硫代谢的酶编码基因在天然红树林生态系统的根际中占主导地位。据我们所知，这是毛里求斯首次对红树林微生物组进行的宏转录组学研究，我们的结果首次提供了有关当地红树林物种功能和微生物多样性的见解。
