Nakao K, Sugawara A, Shiono S, Saito Y, Morii N, Yamada T, Itoh H, Mukoyama M, Arai H, Sakamoto M
Department of Medicine, Kyoto University School of Medicine, Japan.
Can J Physiol Pharmacol. 1987 Aug;65(8):1756-61. doi: 10.1139/y87-275.
To elucidate the secretory form of atrial natriuretic polypeptide from the atrium, the molecular form of atrial natriuretic polypeptide in the perfusate from the isolated beating rat heart and in plasma taken at the coronary sinus of 10 patients during cardiac catheterization has been investigated using high performance gel permeation chromatography and reverse phase high performance liquid chromatography coupled with radioimmunoassay for atrial natriuretic polypeptide. Atrial natriuretic polypeptide in the perfusate from the rat heart showed a single peak eluting at the position of a low molecular weight form of atrial natriuretic polypeptide, without any detectable amounts of atrial natriuretic polypeptide with high molecular weights. The major component of atrial natriuretic polypeptide in the rat heart perfusate co-migrated with rat alpha-atrial natriuretic polypeptide in reverse phase high performance liquid chromatography. In 9 out of 10 patients atrial natriuretic polypeptide in plasma taken at the coronary sinus revealed a single peak of atrial natriuretic polypeptide emerging at the position of human alpha-atrial natriuretic polypeptide in gel filtration. Only one plasma sample had a small quantity of high molecular weight forms with the predominant low molecular weight form of atrial natriuretic polypeptide. The major component of atrial natriuretic polypeptide in the plasma extract from the coronary sinus was identified with human alpha-atrial natriuretic polypeptide. These results indicate that alpha-ANP, a 28-amino acid polypeptide, is secreted as a cardiac hormone into the coronary blood stream from the atrium.
为了阐明心房利钠多肽在心房中的分泌形式，利用高效凝胶渗透色谱法和反相高效液相色谱法结合心房利钠多肽放射免疫分析法，对来自离体搏动大鼠心脏的灌注液以及10例患者在心脏导管插入术期间取自冠状窦的血浆中的心房利钠多肽分子形式进行了研究。大鼠心脏灌注液中的心房利钠多肽在低分子量形式的心房利钠多肽位置洗脱出现一个单峰，未检测到任何高分子量的心房利钠多肽。大鼠心脏灌注液中心房利钠多肽的主要成分在反相高效液相色谱中与大鼠α-心房利钠多肽共迁移。在10例患者中的9例中，取自冠状窦的血浆中的心房利钠多肽在凝胶过滤中显示出在人α-心房利钠多肽位置出现的一个心房利钠多肽单峰。只有一份血浆样本含有少量高分子量形式以及占主导的低分子量形式的心房利钠多肽。来自冠状窦的血浆提取物中心房利钠多肽的主要成分被鉴定为人α-心房利钠多肽。这些结果表明，28个氨基酸的多肽α-ANP作为一种心脏激素从心房分泌到冠状血流中。
