Buyon J P, Shadick N, Berkman R, Hopkins P, Dalton J, Weissmann G, Winchester R, Abramson S B
Department of Medicine, NYU School of Medicine, New York 10003.
Clin Immunol Immunopathol. 1988 Jan;46(1):141-9. doi: 10.1016/0090-1229(88)90014-1.
Complement-derived peptides capable of activating neutrophils appear in plasma during flares of systemic lupus erythematosus (SLE). One possible consequence of such activation is an increased expression of the surface adhesion promoting heterodimer gp165/95 (the complement receptor CR3). The quantity of gp165/95 was measured by indirect immunofluorescence using a monoclonal antibody of the CD11b group. Mol, directed to the alpha chain. Eighty-three percent of 26 patients with SLE expressed gp165/95 on their neutrophil surface to a greater extent than normals. The highest levels of surface gp165/95 were found in patients with the most severe disease, who also had the highest levels of the circulating anaphylatoxin C3a (mean = 560 ng/ml versus 147 ng/ml in controls). There was a negative correlation between expression of gp165/95 and absolute neutrophil count. Five individuals followed serially demonstrated an increase in surface gp165/95 during disease flares which returned to normal with clinical improvement. These data support the hypothesis that the neutrophils of patients with active SLE recruit increased numbers of gp165/95 molecules to their surface in respose to complement activation; these activated neutrophils bearing increased numbers of adhesion promoting gp165/95 may contribute to endothelial injury in SLE.
在系统性红斑狼疮（SLE）发作期间，血浆中会出现能够激活中性粒细胞的补体衍生肽。这种激活的一个可能后果是促进表面黏附的异二聚体gp165/95（补体受体CR3）表达增加。使用针对α链的CD11b组单克隆抗体Mol，通过间接免疫荧光法测定gp165/95的量。26例SLE患者中有83%的中性粒细胞表面gp165/95表达程度高于正常人。在病情最严重的患者中发现表面gp165/95水平最高，这些患者循环中的过敏毒素C3a水平也最高（平均值为560 ng/ml，而对照组为147 ng/ml）。gp165/95的表达与中性粒细胞绝对计数呈负相关。连续随访的5名个体在疾病发作期间表面gp165/95增加，随着临床改善恢复正常。这些数据支持以下假设：活动性SLE患者的中性粒细胞在补体激活反应中会在其表面募集更多数量的gp165/95分子；这些带有更多促进黏附的gp165/95的活化中性粒细胞可能导致SLE中的内皮损伤。
