Song Dan, Zhong Yun, Qian Chunfeng, Zou Qinyan, Ou Jian, Shi Yichao, Gao Liang, Wang Gaigai, Liu Zhenxing, Li Haibo, Ding Hailei, Wu Huihua, Wang Fuxin, Wang Jing, Li Hong
Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
Jiangsu Beike Biotechnology Co., Ltd., Jiangsu 215002, China.
Biomed Res Int. 2016;2016:2517514. doi: 10.1155/2016/2517514. Epub 2016 Mar 7.
Premature ovarian failure (POF) is one of the most common causes of infertility in women. In our present study, we established cyclophosphamide- (CTX-) induced POF rat model and elucidated its effect on ovarian function. We detected the serum estrogen, follicle stimulating hormone, and anti-Müllerian hormone of mice models by ELISA and evaluated their folliculogenesis by histopathology examination. Our study revealed that CTX administration could severely disturb hormone secretion and influence folliculogenesis in rat. This study also detected ovarian cells apoptosis by deoxy-UTP-digoxigenin nick end labeling (TUNEL) and demonstrated marked ovarian cells apoptosis in rat models following CTX administration. In order to explore the potential of human umbilical cord mesenchymal stem cells (UCMSCs) in POF treatment, the above indexes were used to evaluate ovarian function. We found that human UCMSCs transplantation recovered disturbed hormone secretion and folliculogenesis in POF rat, in addition to reduced ovarian cell apoptosis. We also tracked transplanted UCMSCs in ovaries by fluorescence in situ hybridization (FISH). The results manifested that the transplanted human UCMSCs could reside in ovarian tissues and could survive for a comparatively long time without obvious proliferation. Our present study provides new insights into the great clinical potential of human UCMSCs in POF treatment.
卵巢早衰（POF）是女性不孕的最常见原因之一。在我们目前的研究中，我们建立了环磷酰胺（CTX）诱导的POF大鼠模型，并阐明了其对卵巢功能的影响。我们通过ELISA检测了小鼠模型的血清雌激素、卵泡刺激素和抗苗勒管激素，并通过组织病理学检查评估了它们的卵泡发生情况。我们的研究表明，给予CTX会严重干扰大鼠的激素分泌并影响卵泡发生。本研究还通过脱氧尿苷三磷酸地高辛缺口末端标记法（TUNEL）检测了卵巢细胞凋亡，并证明给予CTX后大鼠模型中存在明显的卵巢细胞凋亡。为了探索人脐带间充质干细胞（UCMSCs）在POF治疗中的潜力，我们使用上述指标评估卵巢功能。我们发现，人UCMSCs移植可恢复POF大鼠紊乱的激素分泌和卵泡发生，此外还可减少卵巢细胞凋亡。我们还通过荧光原位杂交（FISH）追踪了卵巢中移植的UCMSCs。结果表明，移植的人UCMSCs可驻留在卵巢组织中，并且可以存活较长时间而无明显增殖。我们目前的研究为人类UCMSCs在POF治疗中的巨大临床潜力提供了新的见解。
