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利用 SLAF-seq 和 SSR 构建花生高密度遗传图谱及控制油酸和亚油酸含量 QTL 的鉴定。

High-Density Genetic Map Construction and Identification of QTLs Controlling Oleic and Linoleic Acid in Peanut using SLAF-seq and SSRs.

机构信息

Shandong Peanut Research Institute, Qingdao, 266100, P.R. China.

Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, P.R. China.

出版信息

Sci Rep. 2018 Apr 3;8(1):5479. doi: 10.1038/s41598-018-23873-7.

DOI:10.1038/s41598-018-23873-7
PMID:29615772
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5883025/
Abstract

The cultivated peanut, A. hypogaea L., is an important oil and food crop globally.High-density genetic linkage mapping is a valuable and effective method for exploring complex quantitative traits. In this context, a recombinant inbred line (RIL) of 146 lines was developed by crossing Huayu28 and P76. We developed 433,679 high-quality SLAFs, of which 29,075 were polymorphic. 4,817 SLAFs were encoded and grouped into different segregation patterns. A high-resolution genetic map containing 2,334 markers (68 SSRs and 2,266 SNPs) on 20 linkage groups (LGs) spanning 2586.37 cM was constructed for peanut. The average distance between adjacent markers was 2.25 cM. Based on phenotyping in seven environments, QTLs for oleic acid (C18:1), linoleic acid (C18:2) and the ratio of oleic acid to linoleic acid (O/L) were identified and positioned on linkage groups A03, A04, A09, B09 and B10. Marker2575339 and Marker2379598 in B09 were associated with C18:1, C18:2 and O/L in seven environments, Marker4391589 and Marker4463600 in A09 were associated with C18:1, C18:2 and O/L in six environments. This map exhibits high resolution and accuracy, which will facilitate QTL discovery for essential agronomic traits in peanut.

摘要

培育的花生，A. hypogaea L.，是全球重要的油料和粮食作物。高密度遗传连锁图谱是探索复杂数量性状的一种有价值和有效的方法。在这种情况下，通过杂交华育 28 和 P76 开发了 146 个系的重组自交系（RIL）。我们开发了 433679 个高质量的 SLAFs，其中 29075 个是多态性的。4817 个 SLAFs 被编码并分为不同的分离模式。构建了一个包含 2334 个标记（68 个 SSR 和 2266 个 SNPs）的花生高分辨率遗传图谱，这些标记位于 20 个连锁群（LG）上，覆盖 2586.37 cM。相邻标记之间的平均距离为 2.25 cM。基于七个环境的表型分析，鉴定了油酸（C18:1）、亚油酸（C18:2）和油酸与亚油酸比值（O/L）的 QTL，并定位在 A03、A04、A09、B09 和 B10 连锁群上。B09 中的标记 2575339 和 Marker2379598 与七个环境中的 C18:1、C18:2 和 O/L 有关，A09 中的标记 4391589 和 Marker4463600 与六个环境中的 C18:1、C18:2 和 O/L 有关。该图谱具有较高的分辨率和准确性，将有助于发现花生重要农艺性状的 QTL。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7650/5883025/f2995fbabefa/41598_2018_23873_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7650/5883025/9de7e4bd5287/41598_2018_23873_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7650/5883025/f2995fbabefa/41598_2018_23873_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7650/5883025/9de7e4bd5287/41598_2018_23873_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7650/5883025/f2995fbabefa/41598_2018_23873_Fig2_HTML.jpg

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