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黄瓜（Cucumis sativus L.）的高密度遗传连锁图谱：基于特定长度扩增片段（SLAF）测序及黄瓜果实性状的QTL分析

A High-Density Genetic Linkage Map for Cucumber (Cucumis sativus L.): Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber.

作者信息

Zhu Wen-Ying, Huang Long, Chen Long, Yang Jian-Tao, Wu Jia-Ni, Qu Mei-Ling, Yao Dan-Qing, Guo Chun-Li, Lian Hong-Li, He Huan-Le, Pan Jun-Song, Cai Run

机构信息

School of Agriculture and Biology, Shanghai Jiaotong University Shanghai, China.

Biomarker Technologies Corporation Beijing, China.

出版信息

Front Plant Sci. 2016 Apr 19;7:437. doi: 10.3389/fpls.2016.00437. eCollection 2016.

DOI:10.3389/fpls.2016.00437
PMID:27148281
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4835494/
Abstract

High-density genetic linkage map plays an important role in genome assembly and quantitative trait loci (QTL) fine mapping. Since the coming of next-generation sequencing, makes the structure of high-density linkage maps much more convenient and practical, which simplifies SNP discovery and high-throughput genotyping. In this research, a high-density linkage map of cucumber was structured using specific length amplified fragment sequencing, using 153 F2 populations of S1000 × S1002. The high-density genetic map composed 3,057 SLAFs, including 4,475 SNP markers on seven chromosomes, and spanned 1061.19 cM. The average genetic distance is 0.35 cM. Based on this high-density genome map, QTL analysis was performed on two cucumber fruit traits, fruit length and fruit diameter. There are 15 QTLs for the two fruit traits were detected.

摘要

高密度遗传连锁图谱在基因组组装和数量性状基因座（QTL）精细定位中起着重要作用。自新一代测序技术出现以来，构建高密度连锁图谱的结构变得更加便捷实用，这简化了单核苷酸多态性（SNP）的发现和高通量基因分型。在本研究中，利用特定长度扩增片段测序技术，以S1000×S1002的153个F2群体构建了黄瓜的高密度连锁图谱。该高密度遗传图谱由3057个特定长度扩增片段（SLAF）组成，包括分布在7条染色体上的4475个SNP标记，图谱跨度为1061.19厘摩（cM）。平均遗传距离为0.35 cM。基于此高密度基因组图谱，对黄瓜的两个果实性状，即果实长度和果实直径进行了QTL分析。共检测到这两个果实性状的15个QTL。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e8/4835494/cb50be3e28e6/fpls-07-00437-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e8/4835494/077ec86283d2/fpls-07-00437-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e8/4835494/304d477b8d9a/fpls-07-00437-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e8/4835494/d7e0432c9ed7/fpls-07-00437-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e8/4835494/13dd47e21f9f/fpls-07-00437-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e8/4835494/cb50be3e28e6/fpls-07-00437-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e8/4835494/077ec86283d2/fpls-07-00437-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e8/4835494/304d477b8d9a/fpls-07-00437-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e8/4835494/d7e0432c9ed7/fpls-07-00437-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e8/4835494/13dd47e21f9f/fpls-07-00437-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12e8/4835494/cb50be3e28e6/fpls-07-00437-g005.jpg

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3
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