胰腺分泌性胰蛋白酶抑制剂基因在肿瘤组织中的表达

Expression of pancreatic secretory trypsin inhibitor gene in neoplastic tissues.

作者信息

Tomita N, Horii A, Yamamoto T, Ogawa M, Mori T, Matsubara K

机构信息

Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.

出版信息

FEBS Lett. 1987 Dec 10;225(1-2):113-9. doi: 10.1016/0014-5793(87)81141-9.

DOI:10.1016/0014-5793(87)81141-9

PMID:2961612

Abstract

Expression of the human pancreatic secretory trypsin inhibitor (PSTI) gene was examined in 24 cases of neoplastic tissues by Northern blot analyses. In three cases of lung adenocarcinoma and one case of sigmoid colon polyp, we detected transcripts which hybridized to the human PSTI cDNA probe. cDNA libraries were constructed using mRNAs of the two PSTI-positive tumor tissues. Two PSTI cDNA clones were obtained from each sample. Sequencing analyses showed that they were completely identical with that of pancreatic PSTI cDNA which had been reported [(1985) Biochem. Biophys. Res. Commun. 132, 605-612]. Southern blot analyses showed that the elevated expression of PSTI in neoplastic tissues was accompanied by neither PSTI gene amplification nor rearrangements.

摘要

通过Northern印迹分析检测了24例肿瘤组织中人胰腺分泌性胰蛋白酶抑制剂（PSTI）基因的表达。在3例肺腺癌和1例乙状结肠息肉中，我们检测到与人类PSTI cDNA探针杂交的转录本。使用两个PSTI阳性肿瘤组织的mRNA构建了cDNA文库。从每个样本中获得了两个PSTI cDNA克隆。测序分析表明，它们与已报道的胰腺PSTI cDNA完全相同[（1985年）《生物化学与生物物理研究通讯》132，605 - 612]。Southern印迹分析表明，肿瘤组织中PSTI的表达升高既没有伴随着PSTI基因扩增，也没有发生重排。