Yamamoto T, Nakamura Y, Nishide J, Emi M, Ogawa M, Mori T, Matsubara K
Biochem Biophys Res Commun. 1985 Oct 30;132(2):605-12. doi: 10.1016/0006-291x(85)91176-3.
We have isolated and sequenced a cDNA clone coding for the human pancreatic secretory trypsin inhibitor (PSTI) from a human pancreatic cDNA library. The predicted product consists of 79 amino acids, and contains no apparent functional polypeptide other than PSTI. Southern blot analysis suggests that there is one copy of PSTI gene per haploid genome. This gene seems to be expressed not only in pancreas, but also in gastric mucosa, since a Northern blot analysis demonstrated the presence of a poly(A) RNA of the same size as in the pancreas. A comparison of sequences between human PSTI mRNA and mouse epidermal growth factor (EGF) mRNA revealed a high homology, suggesting that they share a common ancestral DNA sequence.
我们从人胰腺cDNA文库中分离并测序了一个编码人胰腺分泌型胰蛋白酶抑制剂（PSTI）的cDNA克隆。预测产物由79个氨基酸组成，除PSTI外不包含其他明显的功能性多肽。Southern印迹分析表明，单倍体基因组中PSTI基因有一个拷贝。该基因似乎不仅在胰腺中表达，在胃黏膜中也有表达，因为Northern印迹分析显示存在与胰腺中大小相同的多聚腺苷酸RNA。人PSTI mRNA与小鼠表皮生长因子（EGF）mRNA序列比较显示出高度同源性，表明它们共享一个共同的祖先DNA序列。
