Izumoto Y, Sato T, Yamamoto T, Yoshida N, Kikuchi N, Ogawa M, Matsubara K
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Gene. 1987;59(2-3):151-9. doi: 10.1016/0378-1119(87)90323-4.
Human pancreatic secretory trypsin inhibitor (PSTI) cDNA was expressed in Saccharomyces cerevisiae using the yeast acid phosphatase PHO5 promoter. The product encoded by the PSTI-coding cDNA was correctly processed in yeast cells, and the PSTI molecules were efficiently secreted into the medium. The amino acid composition and the N-terminal amino acid sequence of the secreted PSTI molecules were identical to those of the authentic PSTI polypeptides from human pancreas, and the product exhibited trypsin-inhibitory activity.
利用酵母酸性磷酸酶PHO5启动子，将人胰腺分泌型胰蛋白酶抑制剂（PSTI）cDNA在酿酒酵母中进行表达。PSTI编码cDNA所编码的产物在酵母细胞中得到了正确加工，并且PSTI分子被有效分泌到培养基中。所分泌的PSTI分子的氨基酸组成和N端氨基酸序列与人胰腺中天然PSTI多肽的氨基酸组成和序列相同，并且该产物具有胰蛋白酶抑制活性。
