Tan F, Gippner C, Fink E
Abteilung für Klinische Chemie und Klinische Biochemie, Chirurgischen Klinik Innenstadt, Universität München.
Biol Chem Hoppe Seyler. 1988 May;369 Suppl:51-4.
Oligonucleotide probes were synthesized according to the published cDNA sequence of human pancreatic secretory trypsin inhibitor (PSTI) and used for the screening of a cosmid library of human genomic DNA. One positive clone was isolated, and a subclone obtained after cleavage with a restriction endonucleae (BamHI) was sequenced. The subcloned fragment contained two exons separated by one intron and parts of the two other introns flanking the exons. The exons are encoding five amino acids of the signal peptide and the subsequent 42 N-terminal amino acids of the human PSTI. The available data allow the conclusion that the arrangement of exons and introns in the human PSTI gene is completely identical to that of the chicken ovomucoid and chicken ovoinhibitor genes.
根据已发表的人胰腺分泌型胰蛋白酶抑制剂（PSTI）的cDNA序列合成寡核苷酸探针，并用于筛选人基因组DNA的黏粒文库。分离出一个阳性克隆，用限制性内切酶（BamHI）切割后获得的亚克隆进行测序。亚克隆片段包含两个外显子，中间由一个内含子隔开，以及外显子两侧另外两个内含子的部分序列。这些外显子编码信号肽的五个氨基酸以及人PSTI随后的42个N端氨基酸。现有数据表明，人PSTI基因中外显子和内含子的排列与鸡卵类黏蛋白和鸡卵抑制剂基因的排列完全相同。
