Mohanty Jatindra Nath, Joshi Raj Kumar
Functional Genomics Laboratory, Centre for Biotechnology, Siksha O Anusandhan University, Bhubaneswar, Odisha 751003 India.
3 Biotech. 2018 Mar;8(3):150. doi: 10.1007/s13205-018-1176-4. Epub 2018 Feb 26.
The repertoire and functions of MADS-box family transcription factors (TFs) largely remains unexplored with respect to floral organogenesis of Roxb. Degenerative PCR followed by rapid amplification of cDNA ends was employed in the present study to clone and characterize 17 MADS-box genes (designated as to ) from the floral buds of The cloned genes were clustered into three subgroups (11 MIKC, 4 MIKC* and 2 Mα) based on phylogenetic relationships with the MADS-box genes from and . Southern hybridization showed that all the isolated genes were represented by single copy locus in the genome. Gene structure analysis revealed 1-8 exons in -box genes with the number of exons in MIKC greatly exceeding from that in M-type genes. Motif elicitation of the -box genes indicated the presence of additional domains with MIKC type, suggesting that they had more complex structures. Expression analysis of genes in six transcriptome suggested that, 11 MIKC-type genes are associated with floral homeotic functions, 4 MIKC*-type genes ( to ) controlled the growth of male gametophyte, while the two -type genes ( and ) played significant role in female gametogenesis and seed development. Overall, these are the first set of MADS-box genes from exhibiting a differential expression pattern during floral development. The results from this study will provide valuable information for further functional studies of candidate MADS-box genes in the sexual dimorphism of this economically important dioecious cucurbit.
关于罗汉果花器官发生，MADS盒家族转录因子（TFs）的组成和功能在很大程度上仍未得到探索。本研究采用简并PCR结合cDNA末端快速扩增技术，从罗汉果花芽中克隆并鉴定了17个MADS盒基因（命名为 至 ）。根据与拟南芥和金鱼草MADS盒基因的系统发育关系，将克隆的基因分为三个亚组（11个MIKC、4个MIKC和2个Mα）。Southern杂交显示，所有分离的基因在罗汉果基因组中均以单拷贝位点存在。基因结构分析表明，MADS盒基因含有1 - 8个外显子，MIKC中的外显子数量大大超过M型基因。MADS盒基因的基序分析表明，MIKC型存在额外的结构域，表明它们具有更复杂的结构。对罗汉果六个转录组中基因的表达分析表明，11个MIKC型基因与花同源功能相关，4个MIKC型基因（ 至 ）控制雄配子体的生长，而两个Mα型基因（ 和 ）在雌配子发生和种子发育中起重要作用。总体而言，这些是罗汉果中第一组在花发育过程中表现出差异表达模式的MADS盒基因。本研究结果将为进一步研究这些重要经济作物罗汉果性二态性中候选MADS盒基因的功能提供有价值的信息。
