Department of Pediatrics, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Graduate Institute of Clinical Medical Science, Chang Gung University College of Medicine, Kaohsiung, Taiwan.
Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan.
J Nutr Biochem. 2020 Jan;75:108260. doi: 10.1016/j.jnutbio.2019.108260. Epub 2019 Oct 27.
A maternal high-fat (HF) diet sensitizes offspring to the adverse effects of postnatal HF intake and can lead to metabolic dysregulation. Resveratrol, a natural polyphenolic compound found in grapes and red wine, could help to relieve metabolic syndrome dysregulation. Since the gut microbiota is known to be closely related to metabolic homeostasis, this study aimed to investigate the impact of a combination of maternal and postweaning HF diets on the gut microbiota and whether resveratrol could relieve the gut dysbiosis associated with metabolic dysregulation. Sprague-Dawley dams were sustained on either a chow or HF diet before mating, during pregnancy and during lactation. Their offspring were randomly fed chow or a HF diet after weaning. Four experimental groups were generated: CC (maternal/postnatal chow diet), HC (maternal HF/postnatal chow diet), CH (maternal chow/postnatal high-fat diet) and HH (maternal/postnatal HF diet). A fifth group consisted of HH with resveratrol treatment. We found that both maternal and postnatal HF exposure has a distinct effect on the gut microbiota metagenome of offspring. Maternal HF diet exposure decreased plasma acetate, propionate and butyrate level, while postnatal HF diet exposure decreased plasma acetate level in adult life. The metabolic dysregulation programed by the maternal and postnatal HF diets was related to the relevant gut microbiota. Resveratrol treatment ameliorated the altered plasma propionate level related to maternal HF and postnatal HF diet treatment. Resveratrol treatment also improved most of the altered metabolic dysregulation and related dysbiosis programmed by maternal and postnatal HF diet exposure.
高脂肪（HF）饮食会使母体敏感化，使后代对产后 HF 摄入的不良影响更加敏感，并可能导致代谢失调。白藜芦醇是一种天然存在于葡萄和红酒中的多酚化合物，可能有助于缓解代谢综合征失调。由于肠道微生物群与代谢稳态密切相关，因此本研究旨在研究母体和断奶后 HF 饮食的组合对肠道微生物群的影响，以及白藜芦醇是否可以缓解与代谢失调相关的肠道失调。在交配前、怀孕和哺乳期，斯普拉格-道利母鼠接受标准饮食或 HF 饮食。它们的后代在断奶后随机接受标准饮食或 HF 饮食。生成了四个实验组：CC（母体/产后标准饮食）、HC（母体 HF/产后标准饮食）、CH（母体标准饮食/产后 HF 饮食）和 HH（母体/产后 HF 饮食）。第五组由 HH 与白藜芦醇处理组成。我们发现母体和产后 HF 暴露对后代的肠道微生物群元基因组都有明显的影响。母体 HF 饮食暴露降低了血浆中的乙酸盐、丙酸盐和丁酸盐水平，而产后 HF 饮食暴露降低了成年期的血浆乙酸盐水平。母体和产后 HF 饮食暴露引起的代谢失调与相关的肠道微生物群有关。白藜芦醇处理改善了与母体 HF 和产后 HF 饮食处理相关的改变的血浆丙酸盐水平。白藜芦醇处理还改善了大部分由母体和产后 HF 饮食暴露引起的改变的代谢失调和相关的肠道失调。
