Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG, Brazil.
Department of Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Oxid Med Cell Longev. 2018 Feb 12;2018:7457054. doi: 10.1155/2018/7457054. eCollection 2018.
This study aims to evaluate the effects of a high-fat diet and mechanical ventilation on the pulmonary and systemic inflammatory response in C57BL/6 mice. Male C57BL/6 mice were divided into two groups: one received a standard diet, and the other received a high-fat diet. After 10 weeks, the groups were further divided into two groups each: control group (CG), mechanical ventilation group (MVG), diet group (DG), and diet mechanical ventilation group (DMVG). MVG and DMVG underwent mechanical ventilation for 60 minutes. All animals were euthanized for subsequent analysis. Animals receiving a high-fat diet presented higher body mass, adipose index, and greater adipocyte area. In the lung, the expression of HMGB1 was greater in DG and DMVG than in CG and MVG. CCL2 and IL-22 levels in MVG and DMVG were increased compared to those in CG and DG, whereas IL-10 and IL-17 were decreased. Superoxide dismutase activity was higher in MVG and DMVG than in CG. Catalase activity was lower in DG than in CG, and in MV groups, it was lower than that in CG and DG. MV and obesity promote inflammation and pulmonary oxidative stress in adult C57BL/6 mice.
本研究旨在评估高脂肪饮食和机械通气对 C57BL/6 小鼠肺部和全身炎症反应的影响。雄性 C57BL/6 小鼠分为两组：一组给予标准饮食，另一组给予高脂肪饮食。10 周后，两组进一步分为每组两组：对照组（CG）、机械通气组（MVG）、饮食组（DG）和饮食机械通气组（DMVG）。MVG 和 DMVG 接受机械通气 60 分钟。所有动物均被安乐死以进行后续分析。接受高脂肪饮食的动物体重、脂肪指数和脂肪细胞面积均增加。在肺部，DG 和 DMVG 中 HMGB1 的表达高于 CG 和 MVG。MVG 和 DMVG 中的 CCL2 和 IL-22 水平高于 CG 和 DG，而 IL-10 和 IL-17 水平降低。MVG 和 DMVG 中的超氧化物歧化酶活性高于 CG。DG 中的过氧化氢酶活性低于 CG，MV 组中的过氧化氢酶活性低于 CG 和 DG。MV 和肥胖会促进成年 C57BL/6 小鼠的炎症和肺部氧化应激。
