Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
FASEB J. 2018 Sep;32(9):4878-4888. doi: 10.1096/fj.201700614R. Epub 2018 Apr 5.
Food withdrawal as a health-enhancing measure has beneficial effects on aging, disease prevention, and treatment. However, the cellular and molecular mechanisms involving gut microbial changes and metabolic consequences resulting from food withdrawal have yet to be elucidated. In this study, we subjected lean and obese mice to a dietary intervention that consisted of a 4-d complete food withdrawal and an 8-d 50% food withdrawal, and we studied changes in cecal microbiome and host serum metabolome. The abundance of potentially pathogenic Proteobacteria was decreased and Akkermansia muciniphila was elevated by food withdrawal in mice fed a high-fat diet (HFD). Meanwhile, food withdrawal decreased the abundance of metabolites in branched chain amino acid, lipid, and free fatty acid metabolisms in host serum, more so in HFD mice than in normal mice. Microbial predicted function also showed that food withdrawal decreased the abundance of microbes associated with predicted diseases in the HFD group but not in the normal chow group. Correlation between the microbiome data and metabolomics data revealed a strong association between gut microbial and host metabolic changes in response to food withdrawal. In summary, our results showed that food withdrawal was safer and more metabolically beneficial to HFD-induced obese mice than to normal lean mice, and the beneficial effects were primarily derived from the changes in gut microbiota, which were closely associated with the host metabolome.-Zheng, X., Zhou, K., Zhang, Y., Han, X., Zhao, A., Liu, J., Qu, C., Ge, K., Huang, F., Hernandez, B., Yu, H., Panee, J., Chen, T., Jia, W., Jia, W. Food withdrawal alters the gut microbiota and metabolome in mice.
断食作为一种增进健康的措施，对衰老、疾病预防和治疗都有有益的影响。然而，涉及肠道微生物变化和断食引起的代谢后果的细胞和分子机制尚不清楚。在这项研究中，我们让瘦鼠和肥胖鼠接受了一种饮食干预，包括为期 4 天的完全禁食和为期 8 天的 50%食物摄入。我们研究了盲肠微生物组和宿主血清代谢组的变化。高脂肪饮食（HFD）喂养的小鼠中，断食可降低潜在致病的变形菌门的丰度并增加阿克曼氏菌属的丰度。同时，断食降低了宿主血清中支链氨基酸、脂质和游离脂肪酸代谢物的丰度，在 HFD 小鼠中比在正常饮食小鼠中更为明显。微生物预测功能也表明，断食降低了与 HFD 组预测疾病相关的微生物丰度，但在正常饮食组中没有。微生物组数据和代谢组数据的相关性表明，肠道微生物和宿主对断食的代谢变化之间存在很强的关联。总之，我们的研究结果表明，与正常饮食的瘦鼠相比，断食对 HFD 诱导的肥胖鼠更安全、更有益于代谢，其有益作用主要来源于肠道微生物群的变化，而这种变化与宿主代谢组密切相关。
