Tyrka M, Oleszczuk S, Rabiza-Swider J, Wos H, Wedzony M, Zimny J, Ponitka A, Ślusarkiewicz-Jarzina A, Metzger R J, Baenziger P S, Lukaszewski A J
1Department of Biotechnology and Bioinformatics, Rzeszow University of Technology, Rzeszow, Poland.
2Institute of Plant Breeding and Acclimatization, National Research Institute, Radzikow, Poland.
Mol Breed. 2018;38(4):46. doi: 10.1007/s11032-018-0804-3. Epub 2018 Mar 30.
To create a framework for genetic dissection of hexaploid triticale, six populations of doubled haploid (DH) lines were developed from pairwise hybrids of high-yielding winter triticale cultivars. The six populations comprise between 97 and 231 genotyped DH lines each, totaling 957 DH lines. A consensus genetic map spans 4593.9 cM is composed of 1576 unique DArT markers. The maps reveal several structural rearrangements in triticale genomes. In preliminary tests of the populations and maps, markers specific to wheat segments of the engineered rye chromosome 1R (RM1B) were identified. Example QTL mapping of days to heading in cv. Krakowiak revealed loci on chromosomes 2BL and 2R responsible for extended vernalization requirement, and candidate genes were identified. The material is available to all parties interested in triticale genetics.
为了创建一个用于六倍体小黑麦遗传剖析的框架，从高产冬小黑麦品种的成对杂交种中培育了六个双单倍体（DH）系群体。这六个群体每个包含97至231个基因分型的DH系，共计957个DH系。一个跨度为4593.9 cM的共识遗传图谱由1576个独特的DArT标记组成。这些图谱揭示了小黑麦基因组中的一些结构重排。在对群体和图谱的初步测试中，鉴定出了工程化黑麦染色体1R（RM1B）小麦区段特有的标记。对品种Krakowiak抽穗天数的QTL定位示例揭示了2BL和2R染色体上负责延长春化需求的位点，并鉴定出了候选基因。所有对小黑麦遗传学感兴趣的各方均可获取该材料。
