Department of Tropical Parasitology, Medical University of Gdansk, Powstania Styczniowego 9b, 81-519, Gdynia, Poland.
Department of Biotechnology, Bialystok University of Technology, Wiejska 45E, 15-351, Bialystok, Poland.
Appl Microbiol Biotechnol. 2018 Jun;102(11):4629-4640. doi: 10.1007/s00253-018-8927-3. Epub 2018 Apr 6.
The CRISPR/Cas9 system, a natural defence system of bacterial organisms, has recently been used to modify genomes of the most important protozoa parasites. Successful genome manipulations with the CRISPR/Cas9 system are changing the present view of genetics in parasitology. The application of this system offers a major chance to overcome the current restriction in culturing, maintaining and analysing protozoan parasites, and allows dynamic analysis of parasite genes functions, leading to a better understanding of pathogenesis. CRISPR/Cas9 system will have a significant influence on the process of developing novel drugs and treatment strategies against protozoa parasites.
CRISPR/Cas9 系统是细菌的一种天然防御系统，最近已被用于修改最重要的原生动物寄生虫的基因组。CRISPR/Cas9 系统的成功基因组操作正在改变寄生虫学中遗传学的现有观点。该系统的应用提供了一个克服培养、维持和分析原生动物寄生虫的当前限制的重大机会，并允许对寄生虫基因功能进行动态分析，从而更好地了解发病机制。CRISPR/Cas9 系统将对开发针对原生动物寄生虫的新型药物和治疗策略的过程产生重大影响。
