State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, China.
State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, China.
Int J Biol Macromol. 2018 Jul 15;114:1049-1055. doi: 10.1016/j.ijbiomac.2018.04.004. Epub 2018 Apr 4.
In vivo an ecological network of polysaccharides utilization by gut microbiota is not only an intense competition but also an impressive cooperation pattern. The present study evaluated the in vivo protective effect of combined fungal polysaccharides (CFP) from Cordyceps sinensis and Ganoderma atrum on colon immune dysfunction, induced by 150mg/kg cyclophosphamide (CP). The results showed that C. sinensis polysaccharides (CSP) significantly promoted microbial-derived butyrate to improve histone h3 acetylation mediating regulatory T (Treg) cell specific Foxp3, as well as significantly restored CP-induced elevation of interleukin (IL)-17 and IL-21. Additionally, G. atrum polysaccharides (PSG) significantly down-regulated MyD88, as well as significantly increased IL-10 and TGF-β3. Furthermore, CFP balanced the disequilibrium of cytokines secretion and Foxp3/RORγt ratio related Treg/T helper 17 (Th17) balance, as well as down-regulated the TLR-mediated inflammatory signaling pathway and promoted secretory immunoglobulin A (sIgA) secretion to suppress colonic inflammation. Therefore, our results typically contribute to understand the in vivo immunoregulatory function of fungal polysaccharides compounds, involving microbial-associated inflammatory signals and specific metabolic products.
肠道微生物利用多糖的生态网络不仅是激烈的竞争，也是令人印象深刻的合作模式。本研究评估了蛹虫草和赤芝复合真菌多糖（CFP）对 150mg/kg 环磷酰胺（CP）诱导的结肠免疫功能障碍的体内保护作用。结果表明，虫草多糖（CSP）显著促进微生物衍生的丁酸盐，改善组蛋白 h3 乙酰化介导的调节性 T（Treg）细胞特异性 Foxp3，以及显著恢复 CP 诱导的白细胞介素（IL）-17 和 IL-21 的升高。此外，赤芝多糖（PSG）显著下调 MyD88，显著增加 IL-10 和 TGF-β3。此外，CFP 平衡了细胞因子分泌的失衡和与 Treg/Th17 平衡相关的 Foxp3/RORγt 比值，下调 TLR 介导的炎症信号通路，促进分泌型免疫球蛋白 A（sIgA）分泌，抑制结肠炎症。因此，我们的研究结果有助于理解真菌多糖化合物的体内免疫调节功能，涉及微生物相关的炎症信号和特定的代谢产物。
