Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
J Photochem Photobiol B. 2018 May;182:71-76. doi: 10.1016/j.jphotobiol.2018.03.023. Epub 2018 Mar 30.
The objective of this study was to investigate the therapeutic effect of Folic Acid-Conjugated polyglycerol coated iron oxide nanoparticles on the radiosensitivity of HeLa cells when irradiated with 6 MeV electron beams.
Different concentrations of iron oxide nanoparticles (PG-SPIONs and FA-PG-SPIONs (25, 50, 100, 200 μg ml)) were synthesized by the thermal decomposition technique. The effect of PG-SPIONs and FA-PG-SPIONs in combination with radiation (2, 4, 6 Gy) on the viability of cells and cell survival were estimated using the trypan blue dye exclusion test and MTT assay immediately and 48 h after irradiations, respectively.
It was observed that the penetration rate of uptake for cells treated with >50 μg ml FA-PG-SPIONs was more than that of non-targeted nanoparticles. The data obtained by trypan blue dye exclusion test showed no significant reduction in cell viability for all groups in comparison with control group. The results revealed that increasing the radiation doses in the presence of the concentrations of the nanoparticles increased the value of radiosensitivity. The most radiosensitivity was obtained at the highest concentration of FA-PG-SPIONs (200 μg ml) as well as the longest radiation doses.
It was revealed that higher concentrations of the FA-PG-SPIONs in combination with 6 MeV electron beams could enhance radiosensitization of HeLa cells.
本研究旨在探讨叶酸偶联聚甘油包裹的氧化铁纳米粒子（FA-PG-SPIONs）在 6 MeV 电子束照射下对 HeLa 细胞放射敏感性的影响。
采用热分解法合成不同浓度的氧化铁纳米粒子（PG-SPIONs 和 FA-PG-SPIONs（25、50、100、200 μg/ml）。采用台盼蓝排斥试验和 MTT 法分别在照射后即刻和 48 小时，检测 PG-SPIONs 和 FA-PG-SPIONs 联合辐射（2、4、6 Gy）对细胞活力和细胞存活的影响。
观察到细胞摄取的 FA-PG-SPIONs 浓度大于 50μg/ml 时，穿透率增加。与对照组相比，台盼蓝排斥试验结果显示，所有组的细胞活力均无明显降低。结果表明，在存在纳米粒子浓度的情况下，增加辐射剂量可提高放射敏感性。在 FA-PG-SPIONs 浓度最高（200μg/ml）和辐射剂量最长的情况下，放射敏感性最高。
结果表明，较高浓度的 FA-PG-SPIONs 联合 6 MeV 电子束可增强 HeLa 细胞的放射增敏作用。
