Xinjiang Key Laboratory of Environmental Pollution and Bioremediation, Chinese Academy of Sciences, Urumqi 830011, PR China; College of Environment, Zhejiang University of Technology, Hangzhou 310032, PR China.
College of Environment, Zhejiang University of Technology, Hangzhou 310032, PR China.
Pestic Biochem Physiol. 2018 Apr;146:90-96. doi: 10.1016/j.pestbp.2018.03.002. Epub 2018 Mar 8.
A better knowledge of the intertwined effects of herbicides on plant physiology and microbiome as well as nutrient biogeochemical cycles are needed for environmental management. Here we studied the influence of herbicide diclofop-methyl (DM) on the rice root microbiome and its relationship with N cycle. To do so, we exposed rice seedlings to 100 μg/L DM and studied rhizosphere microbiota using MiSeq-pyrosequencing, root exudation by GC-MS, and denitrification activity by N isotope-tracing and qRT-PCR. The richness and diversity of rhizosphere microorganisms, significantly increased after DM exposure combined with an increase in root exudation of amino acids, sugars, and fatty acids. Transcription of denitrification-related gene and denitrification rate increased significantly in the rice rhizosphere. Our results suggest that DM strongly influenced the root exudation of bacteria nutrients, which affected root microbiome community and potentially influenced N cycle in rice rhizosphere.
为了进行环境管理，我们需要更好地了解除草剂对植物生理学和微生物组以及养分生物地球化学循环的交织影响。在这里，我们研究了除草剂甲羧除草醚（DM）对水稻根系微生物组及其与氮循环关系的影响。为此，我们将水稻幼苗暴露于 100μg/L DM 下，并使用 MiSeq-pyrosequencing、GC-MS 研究根际微生物群落，通过 N 同位素示踪和 qRT-PCR 研究反硝化活性。DM 暴露后，根际微生物的丰富度和多样性显著增加，同时氨基酸、糖和脂肪酸的根分泌物也增加。水稻根际中与反硝化相关的基因转录和反硝化速率显著增加。我们的结果表明，DM 强烈影响了细菌养分的根分泌物，这影响了根际微生物群落，并可能影响了水稻根际中的氮循环。
