Storbeck Karl-Heinz, Gilligan Lorna, Jenkinson Carl, Baranowski Elizabeth S, Quanson Jonathan L, Arlt Wiebke, Taylor Angela E
Department of Biochemistry, Stellenbosch University, Stellenbosch 7602, South Africa; Institute of Metabolism and Systems Research, University of Birmingham, Birmingham B15 2TT, United Kingdom.
Institute of Metabolism and Systems Research, University of Birmingham, Birmingham B15 2TT, United Kingdom.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 May 15;1085:36-41. doi: 10.1016/j.jchromb.2018.03.033. Epub 2018 Mar 20.
Liquid chromatography tandem mass spectrometry (LC-MS/MS) assays are considered the reference standard for serum steroid hormone analyses, while full urinary steroid profiles are only achievable by gas chromatography (GC-MS). Both LC-MS/MS and GC-MS have well documented strengths and limitations. Recently, commercial ultra-high performance supercritical fluid chromatography-tandem mass spectrometry (UHPSFC-MS/MS) systems have been developed. These systems combine the resolution of GC with the high-throughput capabilities of UHPLC. Uptake of this new technology into research and clinical labs has been slow, possibly due to the perceived increase in complexity. Here we therefore present fundamental principles of UHPSFC-MS/MS and the likely applications for this technology in the clinical research setting, while commenting on potential hurdles based on our experience to date.
液相色谱串联质谱（LC-MS/MS）分析被认为是血清类固醇激素分析的参考标准，而完整的尿类固醇谱只有通过气相色谱（GC-MS）才能实现。LC-MS/MS和GC-MS都有充分记录的优点和局限性。最近，已经开发出了商业超高效超临界流体色谱-串联质谱（UHPSFC-MS/MS）系统。这些系统将GC的分辨率与UHPLC的高通量能力结合在一起。这项新技术在研究和临床实验室中的应用进展缓慢，可能是因为人们认为其复杂性增加了。因此，在此我们介绍UHPSFC-MS/MS的基本原理以及该技术在临床研究环境中的可能应用，同时根据我们目前的经验对潜在障碍进行评论。
