Department of Chemical Engineering, Faculty of Engineering & Technology, University of Mazandaran, Babolsar, Iran.
Department of Chemical Engineering, Faculty of Engineering & Technology, University of Mazandaran, Babolsar, Iran.
Carbohydr Polym. 2018 Jun 15;190:281-294. doi: 10.1016/j.carbpol.2018.02.083. Epub 2018 Mar 21.
A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering. Scanning electron microscopy showed Dex/nBGC scaffolds were consisting of a porous 3D microstructure with an average pore size of 240 μm. Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents. It was found that the osmotic pressure and nBGC agglomeration at higher nBGC contents leads to increased water uptake, then reduction of the compressive modulus. Bioactivity of Dex/nBGC scaffolds was validated through apatite formation after submersion in the simulated body fluid. Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks. Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.
一系列由葡聚糖（Dex）和溶胶-凝胶衍生的生物活性玻璃陶瓷纳米粒子（nBGC：0-16（wt%））组成的纳米复合支架被制备为用于骨组织工程的生物活性支架。扫描电子显微镜显示，Dex/nBGC 支架由具有平均孔径为 240μm 的多孔 3D 微结构组成。能谱分析表明，在低 nBGC 含量（2wt%）下，nBGC 纳米粒子均匀分布在 Dex 基质中，而在较高 nBGC 含量下则观察到团聚。研究发现，较高 nBGC 含量下的渗透压和 nBGC 团聚导致吸水率增加，随后压缩模量降低。通过在模拟体液中浸泡后的磷灰石形成来验证 Dex/nBGC 支架的生物活性。结果发现，Dex/nBGC 复合支架在两周内随着 nBGC 含量的增加至 16（wt%），对人成骨细胞（HOBs）的增殖和碱性磷酸酶（ALP）活性有明显的促进作用。由于具有良好的物理化学和生物活性特性，Dex/nBGC 复合水凝胶可用作有前途的骨组织工程应用的生物活性支架。
