Department of Biochemistry and Molecular Biology, Federal University of Parana, CEP 81531-980, Curitiba, PR, Brazil.
Department of Cellular Biology, Federal University of Parana, CEP 81531-980, Curitiba, PR, Brazil.
Carbohydr Polym. 2018 Jun 15;190:50-56. doi: 10.1016/j.carbpol.2018.02.063. Epub 2018 Feb 23.
An aqueous extract containing polysaccharides was obtained from the giant mushroom Macrocybe titans, and it was purified by amylase treatment, freeze-thawing process and dialysis. The purified fraction (ESP) was analyzed by HPSEC and GC-MS which showed a homogenous polysaccharide with M 14.2 × 10 g/mol composed by galactose and fucose. NMR and methylation analysis of ESP confirmed the presence of a fucogalactan with a (1 → 6)-linked α-d-Galp main chain partially substituted at O-2 by non reducing end units of α-l-Fucp residues in the side chain. Its biological activity was evaluated against murine melanoma cells B16-F10. The fucogalactan did not alter the viability, proliferative capacity and morphology of cells. However, this polysaccharide was able to reduce the cell migration in vitro at 40% (100 μg/mL) and 33% (250 μg/mL). The results obtained showed that Macrocybe titans fucogalactan is a promising agent capable of altering melanoma cell migration without decrease the cell viability.
从巨型蘑菇 Macrocybe titans 中获得了一种含有多糖的水提物，并通过淀粉酶处理、冻融和透析进行了纯化。纯化部分（ESP）通过 HPSEC 和 GC-MS 进行了分析，结果表明该多糖是一种均一的多糖，分子量为 14.2×10⁴g/mol，由半乳糖和岩藻糖组成。ESP 的 NMR 和甲基化分析证实了存在一种岩藻半乳聚糖，其（1→6）-连接的α-d-Galp 主链在 O-2 位被非还原端单元的α-l-Fucp 残基部分取代，这些残基位于侧链中。该多糖的生物活性在体外对小鼠黑色素瘤细胞 B16-F10 进行了评估。该岩藻半乳聚糖不会改变细胞活力、增殖能力和形态。然而，该多糖能够在体外减少 40%（100μg/mL）和 33%（250μg/mL）的细胞迁移。研究结果表明，Macrocybe titans 岩藻半乳聚糖是一种有前途的药物，能够改变黑色素瘤细胞的迁移，而不会降低细胞活力。
