Subhi Hasan, Reza Fazal, Husein Adam, Nurul Asma Abdullah
Conservative Dentistry Unit, School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Biomaterials and Prosthodontics Unit, School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
J Conserv Dent. 2018 Jan-Feb;21(1):21-25. doi: 10.4103/JCD.JCD_86_17.
The aim of this study was to evaluate the cytotoxicity effects of experimental gypsum-based biomaterial prepared with various concentrations of chitosan (Gyp-CHT).
The study was performed using cell viability assay for mitochondrial dehydrogenase activity in stem cells from human exfoliated deciduous teeth (SHED), after 1, 2, and 3 days of exposure to the biomaterial extracts of varying concentrations. Differences in mean cell viability values were assessed by one-way analysis of variance, followed by Dunnett T3 test for multiple comparisons ( < 0.05).
The cell viability to Gyp-CHT in low extract concentrations was statistically similar to that of the control and different from that of high extract concentrations. Gyp-5% CHT showed the highest percentage of cell viability with 110.92%, 108.56%, and 109.11%. The cell viability showed a tendency toward increment with low extract concentration and no constant effect of CHT on cell viability toward higher or lower.
Gyp-CHT biomaterial has no cytotoxic effects on the cultured SHED.
本研究旨在评估用不同浓度壳聚糖制备的实验性石膏基生物材料（Gyp-CHT）的细胞毒性作用。
在人脱落乳牙干细胞（SHED）暴露于不同浓度生物材料提取物1、2和3天后，使用细胞活力测定法检测线粒体脱氢酶活性来进行本研究。通过单因素方差分析评估平均细胞活力值的差异，随后进行Dunnett T3检验进行多重比较（P<0.05）。
低提取物浓度下Gyp-CHT的细胞活力在统计学上与对照组相似，与高提取物浓度下的不同。Gyp-5% CHT的细胞活力百分比最高，分别为110.92%、108.56%和109.11%。细胞活力在低提取物浓度下呈增加趋势，壳聚糖对细胞活力没有向更高或更低的恒定影响。
Gyp-CHT生物材料对培养的SHED没有细胞毒性作用。
