Subhi Hasan, Reza Fazal, Husein Adam, Al Shehadat Saaid Ayesh, Nurul Asma-Abdullah
Conservative Dentistry Unit, School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Biomaterials and Prosthodontics Unit, School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Int J Biomater. 2018 Jul 26;2018:3804293. doi: 10.1155/2018/3804293. eCollection 2018.
Effective pulp capping material must be biocompatible and have the ability to induce dentin bridge formation as well as having suitable physical and mechanical properties; however, many current materials do not satisfy the clinical requirements. This study aimed to assess the physical and mechanical properties of gypsum-based chitosan material (Gp-CT) and to evaluate its effects on cellular properties of stem cells from human exfoliated deciduous teeth (SHED). The experimental material was prepared with different concentrations of chitosan (CT) with or without BMP-2. Then, setting time, compressive strength, and pH were determined. In addition, cell viability, alkaline phosphatase (ALP) activity, and cell attachment were assessed. The setting time, compressive strength, and pH obtained were 4.1-6.6 min, 2.63-5.83 MPa, and 6.5-5.7, respectively. The cell viability to gypsum (Gp) with different CT concentrations was similar to that of the control on day 1 but statistically different from that of Gp alone on day 3. The ALP activity of SHED was significantly higher (p < 0.05) in CT- and BMP-2-containing materials than those in the control and Dycal at days 3 and 14. The scanning electron microscopy (SEM) image revealed that flattened cells were distributed across and adhered to the material surface. In conclusion, Gp-CT material shows promise as a potential material for direct pulp capping.
有效的牙髓盖髓材料必须具有生物相容性，具备诱导牙本质桥形成的能力，同时具有合适的物理和机械性能；然而，目前许多材料并不满足临床要求。本研究旨在评估石膏基壳聚糖材料（Gp-CT）的物理和机械性能，并评价其对人乳牙脱落干细胞（SHED）细胞特性的影响。实验材料由不同浓度的壳聚糖（CT）制备而成，添加或不添加骨形态发生蛋白-2（BMP-2）。然后，测定凝固时间、抗压强度和pH值。此外，评估细胞活力、碱性磷酸酶（ALP）活性和细胞黏附情况。所获得的凝固时间、抗压强度和pH值分别为4.1 - 6.6分钟、2.63 - 5.83兆帕和6.5 - 5.7。不同CT浓度的石膏（Gp）在第1天的细胞活力与对照组相似，但在第3天与单独的Gp有统计学差异。在第3天和第14天，含CT和BMP-2材料中SHED的ALP活性显著高于对照组和Dycal（p < 0.05）。扫描电子显微镜（SEM）图像显示扁平细胞分布于材料表面并黏附其上。总之，Gp-CT材料有望成为一种直接盖髓的潜在材料。
