Jung Yoonsun, Yoon Ji-Young, Dev Patel Kapil, Ma Lan, Lee Hae-Hyoung, Kim Jongbin, Lee Jung-Hwan, Shin Jisun
Department of Pediatric Dentistry, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Korea.
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan, Chungcheongnam-do 31116, Korea.
Nanomaterials (Basel). 2020 Jul 14;10(7):1373. doi: 10.3390/nano10071373.
Nanomaterials can enhance interactions with stem cells for tissue regeneration. This study aimed to investigate the biological effects of tricalcium silicate nanoparticle-containing cement (Biodentine™) during or after setting on stem cells from human exfoliated deciduous teeth (SHED) to mimic clinically relevant situations in which materials are adapted. Specimens were divided into four groups depending on the start of extraction time (during (3, 6 and 12 min) or after setting (24 h)) and extracted in culture medium for 24 h for further physicochemical and biological analysis. After cell viability in serially diluted extracts was evaluated, odontogenic differentiation on SHED was evaluated by ARS staining using nontoxic conditions. A physicochemical analysis of extracts or specimens indicated different Ca ion content, pH, and surface chemistry among groups, supporting the possibility of different biological functionalities depending on the extraction starting conditions. Compared to the 'after setting' group, all 'during setting' groups showed cytotoxicity on SHED. The during setting groups induced more odontogenic differentiation at the nontoxic concentrations compared to the control. Thus, under clinically simulated extract conditions at nontoxic concentrations, Biodentine™ seemed to be a promising odontoblast differentiating biomaterial that is helpful for dental tissue regeneration. In addition, to simulate clinical situations when nanoparticle-containing cement is adjusted, biological effects during setting need to be considered.
纳米材料可以增强与干细胞的相互作用以促进组织再生。本研究旨在调查含硅酸三钙纳米颗粒的水泥（BioDentine™）在凝固期间或之后对人乳牙脱落干细胞（SHED）的生物学效应，以模拟材料应用时的临床相关情况。根据提取时间的开始（凝固期间（3、6和12分钟）或凝固后（24小时））将标本分为四组，并在培养基中提取24小时以进行进一步的物理化学和生物学分析。在评估系列稀释提取物中的细胞活力后，使用无毒条件通过ARS染色评估SHED上的成牙本质细胞分化。提取物或标本的物理化学分析表明各组之间的钙离子含量、pH值和表面化学不同，这支持了根据提取起始条件可能具有不同生物学功能的可能性。与“凝固后”组相比，所有“凝固期间”组均对SHED显示出细胞毒性。与对照组相比，凝固期间组在无毒浓度下诱导更多的成牙本质细胞分化。因此，在无毒浓度的临床模拟提取物条件下，BioDentine™似乎是一种有前途的成牙本质细胞分化生物材料，有助于牙齿组织再生。此外，为了模拟含纳米颗粒水泥调整时的临床情况，需要考虑凝固期间的生物学效应。
