四环素、酸与去污剂混合物、次氯酸钠、乙二胺四乙酸和葡萄糖酸洗必泰对玷污层的去除及抗菌作用的扫描电子显微镜评估：一项研究

A Scanning Electron Microscope Evaluation of Smear Layer Removal and Antimicrobial Action of Mixture of Tetracycline, Acid and Detergent, Sodium Hypochlorite, Ethylenediaminetetraacetic Acid, and Chlorhexidine Gluconate: An Study.

作者信息

Charlie K M, Kuttappa M A, George Liza, Manoj K V, Joseph Bobby, John Nishin K

机构信息

Reader, Department of Conservative Dentistry and Endodontics, Annor Dental College and Hospital, Muvattupuzha, Kerala, India.

Professor, Department of Conservative and Endodontics, Cids, Virajpet, Coorg, Karnataka, India.

出版信息

J Int Soc Prev Community Dent. 2018 Jan-Feb;8(1):62-69. doi: 10.4103/jispcd.JISPCD_379_17. Epub 2018 Feb 22.

OBJECTIVES

The main objective is to evaluate the efficiency in removal of smear layer of mixture of tetracycline, acid and detergent (MTAD), sodium hypochlorite (NaOCl), ethylenediaminetetraacetic acid (EDTA) and chlorhexidine gluconate by scanning electron microscope (SEM) evaluation and also to evaluate the antimicrobial action of the same irrigants against standard culture strains of .

MATERIALS AND METHODS

This study included 60 extracted permanent teeth with single root canal. The sample was categorized into five groups with 12 teeth in each group. Root canals were enlarged till size 40 with K-files. One group was kept as control and irrigated only with saline. Other four groups used 5% NaOCl as irrigant during instrumentation and MTAD, 5% NaOCl, 17% EDTA, and 2% chlorhexidine gluconate as final rinse. Teeth were split and examined under SEM. To test the antibacterial action, the zone of inhibition method using agar plates was used. Obtained data were statistically analyzed by SPSS version 17 (SPSS Inc., Chicago, IL, USA).

RESULTS

MTAD and 17% EDTA removed smear layer from all regions of the root canals. About 5% NaOCl and 2% chlorhexidine gluconate were ineffective in removing the smear layer. The mean zone of inhibition formed by the irrigants was in the following order; MTAD (40.5 mm), 2% chlorhexidine gluconate (29.375 mm), 17% EDTA (24.125 mm), 5% NaOCl (22.125 mm), and saline (zero).

CONCLUSION

MTAD showed high smear layer removal efficacy, but no significant difference was found to that of 17% EDTA. As the dimensions of the zones of inhibition showed, MTAD has got highest antibacterial action against , followed by 2% chlorhexidine gluconate, 17% EDTA, and 5% NaOCl. However, the exact correlation of study results to clinical conditions is impossible due to the variables involved.

目的

主要目的是通过扫描电子显微镜（SEM）评估来评价四环素、酸和洗涤剂混合物（MTAD）、次氯酸钠（NaOCl）、乙二胺四乙酸（EDTA）和葡萄糖酸氯己定去除玷污层的效率，同时评估相同冲洗剂对标准培养菌株的抗菌作用。

材料与方法

本研究纳入60颗单根管的拔除恒牙。样本分为五组，每组12颗牙齿。用K锉将根管扩大至40号。一组作为对照组，仅用生理盐水冲洗。其他四组在根管预备时用5% NaOCl冲洗，并用MTAD、5% NaOCl、17% EDTA和2%葡萄糖酸氯己定进行最终冲洗。将牙齿劈开并在SEM下检查。为测试抗菌作用，采用琼脂平板抑菌圈法。所得数据用SPSS 17版软件（SPSS公司，美国伊利诺伊州芝加哥）进行统计学分析。

结果

MTAD和17% EDTA能去除根管各部位的玷污层。约5% NaOCl和2%葡萄糖酸氯己定去除玷污层无效。冲洗剂形成的平均抑菌圈大小顺序如下：MTAD（40.5毫米）、2%葡萄糖酸氯己定（29.375毫米）、17% EDTA（24.125毫米）、5% NaOCl（22.125毫米）和生理盐水（零）。