Mureşan Carmen I, Schierhorn Angelika, Buttstedt Anja
Institut für Biologie, Molekulare Ökologie , Martin-Luther-Universität Halle-Wittenberg , Hoher Weg 4 , 06120 Halle (Saale) , Germany.
Universitatea de Ştiinţe Agricole şi Medicină Veterinară, Departamentul de Apicultură şi Sericicultură şi Biotehnologii , Calea Mănăştur 3-5 , 400372 Cluj-Napoca , Romania.
J Agric Food Chem. 2018 Apr 25;66(16):4164-4170. doi: 10.1021/acs.jafc.8b00961. Epub 2018 Apr 11.
Royal jelly (RJ) is a beehive product with a complex composition, major royal jelly proteins (MRJPs) being the most abundant proteins. Cell culture and animal studies suggest various biological activities for the full-length/native MRJPs. In the field of apitherapy, it is assumed that MRJPs can positively affect human health. However, whenever RJ is administered orally, the availability for assimilation in the gastrointestinal tract is a prerequisite for MRJPs to have any effect on humans. We here show that MRJPs vary in resistance to pepsin digestion with MRJP2 being most stable and still present as full-length protein after 24 h of digestion. In the intestinal phase, using trypsin and chymotrypsin, MRJPs are rapidly digested with MRJP2 again showing longest stability (40 min), suggesting that MRJPs can reach the small intestine as full-length proteins but then have to be resorbed quickly if full-length proteins are to fulfill any biological activity.
蜂王浆（RJ）是一种成分复杂的蜂箱产品，主要蜂王浆蛋白（MRJPs）是其中含量最丰富的蛋白质。细胞培养和动物研究表明全长/天然MRJPs具有多种生物活性。在蜂疗领域，人们认为MRJPs可以对人体健康产生积极影响。然而，每当口服RJ时，胃肠道中可用于同化的可用性是MRJPs对人类产生任何影响的先决条件。我们在此表明，MRJPs对胃蛋白酶消化的抗性各不相同，其中MRJP2最稳定，消化24小时后仍以全长蛋白形式存在。在肠道阶段，使用胰蛋白酶和胰凝乳蛋白酶时，MRJPs会迅速被消化，MRJP2再次表现出最长的稳定性（40分钟），这表明MRJPs可以以全长蛋白的形式到达小肠，但如果全长蛋白要发挥任何生物活性，则必须迅速被吸收。
