Franzo Giovanni, Legnardi Matteo, Centelleghe Cinzia, Tucciarone Claudia M, Cecchinato Mattia, Cortey Martí, Segalés Joaquim, Drigo Michele
Departments of Animal Medicine, Production and Health (MAPS; Franzo, Legnardi, Tucciarone, Cecchinato, Drigo).
Comparative Biomedicine and Food Science (BCA; Centelleghe).
J Vet Diagn Invest. 2018 Jul;30(4):538-544. doi: 10.1177/1040638718770495. Epub 2018 Apr 9.
Since the identification of species Porcine circovirus 2, the relevance of genus Circovirus has increased given its impact on the swine industry. A new species ( Porcine circovirus 3, PCV-3) has been detected in association with various clinical conditions. Consequently, there is an urgent need for reliable and widely accessible tests for both routine diagnostic and research purposes. We developed a direct PCR (requiring no DNA extraction) and a quantitative (q)PCR targeting the conserved rep gene to detect the PCV-3 genome. Test performance was assessed by testing 120 field samples within different matrices. Both methods were sensitive (detection of 10 viral genome/µL), specific, and repeatable. The substantially perfect agreement between the 2 assays strongly supports their high sensitivity and specificity. The low cost and short processing time of the direct PCR protocol, together with the reliable quantitative results provided by qPCR, support the establishment of common testing guidelines.
自猪圆环病毒2型被鉴定以来，圆环病毒属因其对养猪业的影响而受到越来越多的关注。一种新的病毒种（猪圆环病毒3型，PCV-3）已被检测出与多种临床病症相关。因此，迫切需要开发出可靠且易于获取的检测方法，用于常规诊断和研究。我们开发了一种直接PCR方法（无需进行DNA提取）以及一种针对保守的rep基因的定量（q）PCR方法，用于检测PCV-3基因组。通过对120份来自不同样本基质的田间样本进行检测，评估了这两种检测方法的性能。两种方法均具有敏感性（可检测到10个病毒基因组/微升）、特异性和可重复性。两种检测方法之间高度一致，有力地证明了它们具有高敏感性和特异性。直接PCR方法成本低、处理时间短，同时qPCR能够提供可靠的定量结果，这些都支持建立通用的检测指南。
