Nisavic Jakov, Milic Nenad, Radalj Andrea, Mirilovic Milorad, Vejnovic Branislav, Cosic Milivoje, Knezevic Aleksandra, Veljovic Ljubisa, Zivulj Aleksandar
Department of Microbiology, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia.
Department of Economics and Statistics, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia.
Vet Med (Praha). 2022 Jan 10;67(3):131-137. doi: 10.17221/32/2021-VETMED. eCollection 2022 Mar.
The objective was to expand and update the knowledge on the presence and genotype diversity of porcine circoviruses 2 and 3 (PCV2 and PCV3) in the wild boar populations from the hunting grounds in northeastern Serbia. The presence of PCV3 was not determined, and PCV2 was confirmed in 40.32% of the organ samples from 124 wild boars hunted from 2018 to 2019, indicating their significance in virus circulation since traditional pig farms with irregular PCV2 vaccination strategies are widespread in this region. The most prevalent genotype was PCV2d, followed by PCV2b and PCV2a in 55.6%, 38.9%, and 5.5% of the examined samples, respectively. Nucleotide sequences of the detected strains were homogenous within the genotype and clustered within the subgroups PCV2d-2, PCV2b-1A/B, and PCV2a-2D with high identity to European, Chinese, and Serbian domestic pig sequences suggesting their origin. Wild boars presented with no clinical or pathological signs of infection, implying that these animals might be less susceptible to disease, particularly since the cofactors present in pig farming systems that support the disease development are absent in the wild. The high PCV2 detection frequency demonstrates the importance of wildlife monitoring to track virus population dynamics, especially in regions with free-range pig farming in order to plan adequate disease control strategies.
目的是扩大和更新关于塞尔维亚东北部狩猎场野猪种群中猪圆环病毒2型和3型（PCV2和PCV3）的存在情况及基因型多样性的知识。未检测到PCV3的存在，在2018年至2019年猎杀的124头野猪的器官样本中，40.32%的样本检测到PCV2，这表明它们在病毒传播中具有重要意义，因为该地区存在大量传统养猪场，且PCV2疫苗接种策略不规范。最常见的基因型是PCV2d，其次是PCV2b和PCV2a，分别占检测样本的55.6%、38.9%和5.5%。检测到的毒株的核苷酸序列在基因型内是同源的，并聚集在PCV2d-2、PCV2b-1A/B和PCV2a-2D亚组内，与欧洲、中国和塞尔维亚家猪序列具有高度同源性，表明了它们的起源。野猪没有出现感染的临床或病理症状，这意味着这些动物可能对疾病的易感性较低，特别是因为野猪缺乏养猪系统中支持疾病发展的辅助因素。PCV2的高检测频率表明监测野生动物以追踪病毒种群动态的重要性，特别是在有散养猪的地区，以便制定适当的疾病控制策略。
