中国江苏省自由放养野猪和蜱中猪圆环病毒3型的检测

Detection of Porcine Circovirus Type 3 in Free-Ranging Wild Boars and Ticks in Jiangsu Province, China.

作者信息

Sun Fanqi, Li Meng, Wang Yi, Cheng Wangkun, Li Meirong, Deng Changlin, Wang Xianwei, Yang Zhen

机构信息

Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, The Belt and Road International Sci-Tech Innovation Institute of Transboundary Animal Disease Diagnosis and Immunization, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.

Nanjing Hongshan Forest Zoo, Nanjing 210028, China.

出版信息

Viruses. 2025 Jul 28;17(8):1049. doi: 10.3390/v17081049.

DOI:10.3390/v17081049

PMID:40872764

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12390564/

Abstract

Porcine circovirus type 3 (PCV3) has been detected in wild boars across many countries in Europe, Asia, and South America. However, data regarding the presence of porcine circoviruses in wild boars and ticks remain limited. In this study, we investigated the presence and genetic characteristics of PCV3 in wild boars and parasitizing ticks in Jiangsu, China. Samples, including whole blood, serum, tissues, feces, and oral fluids from wild boars, as well as ticks collected from 47 wild boars, were obtained between March 2021 and November 2022. PCR results indicated that 34.0% (16/47) of wild boars tested positive for PCV3, while ELISA detected 41.9% (18/43) seropositivity. RT-qPCR results showed that 7.2% (6/83) were positive for PCV3 in 83 analyzed tick samples, with all positive samples identified as . The PCV3 genome obtained from wild boars was classified as PCV3a and was closely related to the strain identified in domestic pigs in Nanjing, Jiangsu Province. Collectively, these findings confirm the presence of PCV3 in wild boars in Jiangsu and suggest a possible link of PCV3 infection among domestic pigs, wild boars, and ticks, providing new insights into the transmission risk of PCV3 at wildlife-livestock-human interfaces and highlighting the genetic homology between strains from wild and domestic pigs.

摘要

在欧洲、亚洲和南美洲的许多国家，野猪体内都检测到了3型猪圆环病毒（PCV3）。然而，关于野猪和蜱虫中猪圆环病毒存在情况的数据仍然有限。在本研究中，我们调查了中国江苏省野猪及其寄生蜱虫中PCV3的存在情况和遗传特征。在2021年3月至2022年11月期间，采集了野猪的全血、血清、组织、粪便和口腔分泌物样本，以及从47头野猪身上采集的蜱虫样本。PCR结果显示，34.0%（16/47）的野猪PCV3检测呈阳性，而ELISA检测到41.9%（18/43）的血清阳性。RT-qPCR结果表明，在83份分析的蜱虫样本中，7.2%（6/83）的样本PCV3呈阳性，所有阳性样本均被鉴定为。从野猪身上获得的PCV3基因组被归类为PCV3a，与江苏省南京市家猪中鉴定出的毒株密切相关。总体而言，这些发现证实了江苏省野猪中存在PCV3，并表明PCV3可能在家猪、野猪和蜱虫之间存在感染联系，为PCV3在野生动物-家畜-人类界面的传播风险提供了新的见解，并突出了野生和家猪毒株之间的遗传同源性。