a Department of Orthopedics , Shanghai Jiao Tong University Affiliated Sixth People's Hospital , No.600, Yishan Road, Shanghai , China.
Cancer Biol Ther. 2018 Jul 3;19(7):565-572. doi: 10.1080/15384047.2017.1416273. Epub 2018 Apr 13.
Osteosarcoma (OS) is one of the most common primary bone tumors and has a high disablity rate and case-fatality rate. The protracted stagnancy of the chemotherapy program and surgical technology for OS treatment prompted us to focus on the mechanisms of cancer carcinogenesis progression in OS. Nucleoside diphosphate kinase B (NME2) is a type of nucleoside diphosphate kinase that plays an important role in cellular processes. In this study, we report overexpression of NME2 in OS cell lines and correlate this overexpression with the clinicopathologic features of osteosarcoma. We used si-NME2 to downregulate expression of NME2 in OS cell lines. The results of the CCK8 and clone forming assays show that NME2 promotes OS cell line proliferation. Western blot assays show that deregulation of NME2 results in enhanced the expression of c-Myc, which promotes OS proliferation.
骨肉瘤（OS）是最常见的原发性骨肿瘤之一，具有很高的致残率和病死率。骨肉瘤化疗方案和手术技术的长期停滞促使我们关注骨肉瘤中癌症发生进展的机制。核苷二磷酸激酶 B（NME2）是一种核苷二磷酸激酶，在细胞过程中发挥重要作用。在本研究中，我们报告了 NME2 在骨肉瘤细胞系中的过表达，并将这种过表达与骨肉瘤的临床病理特征相关联。我们使用 si-NME2 下调 OS 细胞系中 NME2 的表达。CCK8 和克隆形成检测结果表明，NME2 促进 OS 细胞系增殖。Western blot 检测结果表明，NME2 的失调导致 c-Myc 的表达增强，从而促进 OS 的增殖。
