Joffin Nolwenn, Jaubert Anne-Marie, Durant Sylvie, Barouki Robert, Forest Claude, Noirez Philippe
Université Paris Descartes, Sorbonne Paris Cité, France.
Institut National de la Santé et de la Recherche Médicale UMR-S 1124, Faculté des Sciences Fondamentales et Biomédicales, Pharmacologie Toxicologie et Signalisation Cellulaire, 45 rue des Saints Pères, 75006 Paris, France.
Biochim Open. 2015 Jun 5;1:1-5. doi: 10.1016/j.biopen.2015.05.001. eCollection 2015.
We recently demonstrated that citrulline (CIT) reduced the expression of inflammatory genes in cultured explants from retroperitoneal (RET) white adipose tissue (WAT) from young (2-4 months) but not old (25 months) rats. Here we show that in RET WAT from old rats and high-fat-diet-fed (HFD) young rats, the basal expression of the leptin gene was increased (275-345%) whereas that of the adiponectin gene was decreased (48-60%), when compared to those from control-diet-fed (CD) young rats. We show also that in RET WAT from old rats, a diet supplemented with CIT for 3 months reduced macrophage (F4/80, CD68) and inflammation (interleukin-6, tumor necrosis factor-α) marker genes 23-97%. CIT supplementation lowered leptin mRNA 62% and increased adiponectin mRNA 232%. In cultured explants of RET WAT from 4 month-old CD, 4 month-old HFD and 25-month-old CD rats, the exposure to 2.5 mmol/L CIT for 24 h up-regulated adiponectin gene expression 151%, 362% and 216% respectively. In contrast, leptin gene expression was down-regulated 66% selectively in CIT-treated explants from 25-month-old CD rats. These results further support the proposed beneficial effect of CIT to counteract the deleterious effects of aging and overweight on the metabolic, inflammatory and endocrine functions of WAT.
我们最近证明，瓜氨酸（CIT）可降低幼年（2 - 4个月）而非老年（25个月）大鼠腹膜后（RET）白色脂肪组织（WAT）培养外植体中炎症基因的表达。在此我们表明，与对照饮食喂养（CD）的幼年大鼠相比，老年大鼠和高脂饮食喂养（HFD）的幼年大鼠的RET WAT中，瘦素基因的基础表达增加（275 - 345%），而脂联素基因的基础表达降低（48 - 60%）。我们还表明，在老年大鼠的RET WAT中，补充CIT 3个月的饮食可使巨噬细胞（F4/80、CD68）和炎症（白细胞介素 - 6、肿瘤坏死因子 - α）标记基因减少23 - 97%。补充CIT可使瘦素mRNA降低62%，脂联素mRNA增加232%。在4个月大的CD、4个月大的HFD和25个月大的CD大鼠的RET WAT培养外植体中，暴露于2.5 mmol/L CIT 24小时分别使脂联素基因表达上调151%、362%和216%。相反，在25个月大的CD大鼠经CIT处理的外植体中，瘦素基因表达选择性下调66%。这些结果进一步支持了CIT对抵消衰老和超重对WAT代谢、炎症和内分泌功能的有害影响所提出的有益作用。
