Di Cerbo Alessandro, Canello Sergio, Guidetti Gianandrea, Fiore Filippo, Corsi Lorenzo, Rubattu Nicola, Testa Cecilia, Cocco Raffaella
Department of Medical, Oral and Biotechnological Sciences, Dental School, University G. D'Annunzio of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
Vet Ital. 2018 Jun 30;54(2):137-146. doi: 10.12834/VetIt.1357.7466.2.
In the last decades, adverse food reactions have increased considerably in dogs and cats. In this study we report on the possible onset of food intolerances symptoms, including otitis, diarrhoea, generalised anxiety, and dermatitis in a cohort of 8 dogs consuming commercial diets. All dogs received an organic chicken-based diet for 15 days. We performed analysis of blood biochemical parameters, kibble composition, and oxytetracycline (OTC) serum concentration before and after 15 days of organic chicken-based diet supplementation. We hypothesised that a chronic intake of contaminated food enhanced by the presence of nanoparticle aggregates might be at the base of the onset of pharmacologic or idiopathic food intolerances. At the end of the evaluation period, an overall significant reduction of otitis, diarrhoea, generalised anxiety, and dermatitis was observed. Biochemical analyses indicate a significant increase in the alkaline phosphatase, from 41 to 52.5 U/L, after 15 days (••p <0.01), while a significant decrease in Gamma-glutamyl transferase and urea, from 9.37 to 6.25 U/L and from 32.13 ± 8.72 to 22.13 ± 7.8 mg/dL, respectively, was observed (•p <0.05). A significant decrease, from 0.22 to 0.02 μg/mL, in mean OTC serum concentration was also observed (••p <0.01). Composition analysis revealed the presence of OTC, calcium, aluminium, silicon, and phosphorous nanoparticle aggregates. Further research on a wider sample size would help to confirm the hypothesis proposed here.
在过去几十年中，犬猫的食物不良反应显著增加。在本研究中，我们报告了一组8只食用商业日粮的犬出现食物不耐受症状的可能情况，这些症状包括中耳炎、腹泻、广泛性焦虑和皮炎。所有犬均接受了15天的有机鸡肉日粮。我们在补充有机鸡肉日粮15天前后，对血液生化参数、干粮成分和土霉素（OTC）血清浓度进行了分析。我们假设，纳米颗粒聚集体的存在导致的受污染食物的长期摄入，可能是药理或特发性食物不耐受发病的根本原因。在评估期结束时，观察到中耳炎、腹泻、广泛性焦虑和皮炎总体上显著减轻。生化分析表明，15天后碱性磷酸酶显著升高，从41 U/L升至52.5 U/L（••p<0.01），而γ-谷氨酰转移酶和尿素分别从9.37 U/L降至6.25 U/L以及从32.13±8.72 mg/dL降至22.13±7.8 mg/dL，均有显著下降（•p<0.05）。平均OTC血清浓度也显著下降，从0.22 μg/mL降至0.02 μg/mL（••p<0.01）。成分分析发现了OTC、钙、铝、硅和磷的纳米颗粒聚集体。对更大样本量的进一步研究将有助于证实本文提出的假设。
